INCENP Antibody

Product Discontinued

INCENP Antibody Summary

• Immunogen: A synthetic peptide derived from a C-terminal sequence of human INCENP. [UniProt# Q9NQS7]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: INCENP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
• Application Notes: This INCENP antibody is useful for Immunocytochemistry/Immunofluorescence. The theoretical molecular weight of human INCENP is ~105.5 kDa. Optimal working dilutions should be determined by the investigator. Western Blot: Not tested; Projected working range is 1:1,000-1:10,000 Immunoprecipitation: Not tested; Projected working range is 5-20 ug/mg lysate Immunohistochemistry: Not tested; Projected working range is 1:100-1:1,000.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 105.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human. Preliminary data suggests reactivity with Xenopus INCENP in immunofluorescence. Immunogen sequence has 84% identity with mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.01% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for INCENP Antibody

• binds and activates aurora-B and -C in vivo and in vitro
• chromosomal passenger protein
• FLJ31633
• inner centromere protein antigens (135kD, 155kD)
• inner centromere protein antigens 135/155kDa
• inner centromere protein INCENP
• inner centromere protein
• MGC111393

Background

INCENP (inner centromere protein) is one of the key components of the CPC complex (BIRC5/survivin, CDCA8/borealin, INCENP, AURKB and AURKC) which implicates in the regulation of mitotic cell division. CPC complex play a critical role at centromeres in facilitating correct chromosome alignment/segregation and is essential for chromatin-induced microtubule stabilization as well as spindle assembly. INCENP has been shown to localize to inner kinetochore and on chromosome arms and inner centromeres from prophase through metaphase and then transferring to spindle midzone and midbody from anaphase through cytokinesis. INCENP exists as a homodimer or heterodimer, interacts with several proteins that includes H2AFZ, CBX3, tubulin beta chain, AURKB/AURKC, EVI5, CDCA8, BIRC5, CBX5; CBX5, POGZ, and JTB. Moreover, INCENP colocalizes with AURKB at mitotic chromosomes where INCENP-AURKB/AURKC association is important for its functions and INCENP also binds directly to microtubules for regulating the kinetochore localization of BUB1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: INCENP
• Entrez:
  • 3619(Human)
• Uniprot:
  • Q9NQS7(Human)