Importin alpha 3/KPNA4 Antibody (843124) [Unconjugated]

Western blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Importin a3/KPNA4 Monoclonal Antibody (Catalog # MAB8204) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Importin a3/KPNA4 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for Importin alpha 3/KPNA4 at approximately 56 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Importin alpha 3/KPNA4 Monoclonal Antibody (Catalog # MAB8204). This experiment was conducted under reducing conditions and using the12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clone: 843124
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Importin alpha 3/KPNA4 Antibody (843124) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Importin alpha 3/KPNA4
  Met1-Asn104
  Accession # O00629
• Specificity: Detects human Importin alpha 3/KPNA4 in ELISA and Western blot. In direct ELISA, no cross-reactivity to human KPNA3 was detected.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: KPNA4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Importin alpha 3/KPNA4 Antibody (843124) [Unconjugated]

• Importin alpha 3
• Importin alpha Q1
• importin subunit alpha-4
• importin-alpha-Q1
• IPOA3
• karyopherin alpha 4 (importin alpha 3)
• KPNA4
• MGC12217
• MGC26703
• Qip1
• QIP12
• QIP1Karyopherin subunit alpha-4
• SRP3

Background

KPNA4 (Karyopherin subunit alpha 4), also called Importin subunit a4 or Importin aQ1 (previously called Qip1 or Importin a3) is an approximately 58-60 kDa member of the Importin alpha family of proteins. It is ubiquitously expressed, and found in both nucleus and cytoplasm. KPNA4 functions as a cargo carrier that transports various complexes from cytoplasm into nucleus.  Human KPNA4 is a 521 amino acid (aa) protein that contains an N-terminal IBB/Importin b domain (aa 2-58), ten Armadillo repeats that bind “cargo” (aa 66-485) and two intervening NLS binding sites. Human KPNA4 aa 1-104 share 99% aa identity with mouse and rat KPNA4, which in turn share 100% identity with each other.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KPNA4
• Uniprot:
  • O00629()