Importin alpha 2/KPNA2 Antibody - BSA Free

Western Blot: Importin alpha 2/KPNA2 Antibody [NB100-79808] - Whole cell lysate (10 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RCH1/KPNA2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody [NB100-79808] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-RCH1/KPNA2 used at 1:5,000 (0.2ug/ml). Detection: DAB

Western Blot: Importin alpha 2/KPNA2 Antibody [NB100-79808] - Whole cell lysate (10 ug) from HEK293T, HeLa, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RCH1/KPNA2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Immunohistochemistry-Paraffin: Importin alpha 2/KPNA2 Antibody [NB100-79808] - FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbitanti-RCH1/KPNA2 used at 1:5,000 (0.2ug/ml). Detection: DAB

Immunoprecipitation: Importin alpha 2/KPNA2 Antibody [NB100-79808] - Detection of human RCH1/KPNA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RCH1/KPNA2 antibody NB100-79808 (lot NB100-79808-2) used for IP at 6 ug per reaction. RCH1/KPNA2 was also immunoprecipitated by a previous lot of this antibody (lot NB100-79808-1) and rabbit anti-RCH1/KPNA2 antibody NB100-79807. For blotting immunoprecipitated RCH1/KPNA2, NB100-79808 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Importin alpha 2/KPNA2 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 475 and the C-terminus (residue 529) of human RAG cohort 1 (Karyopherin Alpha 2) using the numbering given in entry NP_002257.1 (GeneID 3838).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KPNA2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000 -1:5000
  • Immunohistochemistry-Paraffin 1:1000 -1:5000
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:10000 - 1:25000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Control:

  Importin alpha 2/KPNA2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Importin alpha 2/KPNA2 Antibody - BSA Free

• Importin alpha 1
• Importin alpha 2
• importin-alpha-P1
• IPOA1
• karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
• Karyopherin subunit alpha-2
• KPNA2
• Pendulin
• QIP2
• QIP2importin alpha 2
• RAG cohort 1
• RAG cohort protein 1
• RCH1
• RCH1importin subunit alpha-2
• SRP1
• SRP1alpha
• SRP1-alpha

Background

The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Importin alpha 2/KPNA2 Antibody (NB100-79808)(1)

Publication using NB100-79808

• Chatterjee S, Kundu CN Nanoformulated quinacrine regulates NECTIN-4 domain specific functions in cervical cancer stem cells Eur. J. Pharmacol. 2020-06-27 [PMID: 32603697] (IP, Human)

Bioinformatics

• Gene Symbol: KPNA2
• Entrez:
  • 3838(Human)
• Uniprot:
  • NP_002257.1(Human)