Importin alpha 2/KPNA2 Overexpression Lysate (Native)

Images

 
Western Blot: KPNA2 Overexpression Lysate (Adult Normal) [NBL1-12370] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for KPNA2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Importin alpha 2/KPNA2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC208803 and based on accession number NM_002266. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens karyopherin alpha 2 (RAG cohort 1, importin alpha 1) (KPNA2), mRNA.
Gene
KPNA2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
57.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Importin alpha 2/KPNA2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Importin alpha 2/KPNA2 Overexpression Lysate (Native)

  • Importin alpha 1
  • Importin alpha 2
  • importin-alpha-P1
  • IPOA1
  • karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
  • Karyopherin subunit alpha-2
  • KPNA2
  • Pendulin
  • QIP2
  • QIP2importin alpha 2
  • RAG cohort 1
  • RAG cohort protein 1
  • RCH1
  • RCH1importin subunit alpha-2
  • SRP1
  • SRP1alpha
  • SRP1-alpha

Background

The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Importin alpha 2/KPNA2 Lysate (NBL1-12370) (0)

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Bioinformatics

Gene Symbol KPNA2
Entrez