Importin-7 Antibody

Flow Cytometry: Importin-7 Antibody [NB100-1081] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Product Discontinued

Importin-7 Antibody Summary

• Immunogen: Peptide with sequence C-SSFNFGGPAPGMN corresponding to C-Terminus according to NP_006382.1.
• Localization: Nuclear membrane
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IPO7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Knockdown Validated
  • Peptide ELISA Detection limit 1:4000
• Application Notes: WB: Approx. 120 kDa band observed in A431, Daudi and HeLa lysates (calculated MW of 120 kDa band according to NP_006382.1). An additional band of unknown identity was also consistently observed at 26-28 kDa. This band was successfully blocked by incubation with the immunizing peptide.
• Control:

  Human Brain Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Importin-7 Antibody

• FLJ14581
• Imp7MGC138673
• importin 7
• RAN binding protein 7
• Ran-binding protein 7
• RanBP7
• RANBP7importin-7

Background

The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran binding site of importin beta. Similar to importin beta, this protein prevents the activation of Ran's GTPase by RanGAP1 and inhibits nucleotide exchange on RanGTP, and also binds directly to nuclear pore complexes where it competes for binding sites with importin beta and transportin. This protein has a Ran-dependent transport cycle and it can cross the nuclear envelope rapidly and in both directions. At least four importin beta like transport receptors, namely importin beta itself, transportin, RanBP5 and importin 7, directly bind and import ribosomal proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Importin-7 Antibody (NB100-1081)(5)

Publications using NB100-1081

• Braun DA, Sadowski CE, Kohl S et al. Mutations in nuclear pore genes NUP93, NUP205 and XPO5 cause steroid-resistant nephrotic syndrome. Nat Genet 2016-04-01 [PMID: 26878725]
• Saijou E, Itoh T, Kim KW et al. Nucleocytoplasmic shuttling of the zinc finger protein EZI Is mediated by importin-7-dependent nuclear import and CRM1-independent export mechanisms. J Biol Chem. 2007-11-02 [PMID: 17848547]
• Gorlich D, Dabrowski M, Bischoff FR et al. A novel class of RanGTP binding proteins. J Cell Biol 1997-07-14 [PMID: 9214382]
• nataraj N, Noronha A, Sang Lee J et al. Nucleoporin-93 Overcomes Multiple Nucleocytoplasmic Trafficking Bottlenecks and Permits Robust Metastasis SSRN Electronic Journal 2021-03-31
• Nataraj NB, Noronha A, Lee JS et al. Nucleoporin-93 reveals a common feature of aggressive breast cancers: robust nucleocytoplasmic transport of transcription factors Cell reports 2022-02-22 [PMID: 35196484]

Review for Importin-7 Antibody (NB100-1081) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Ariel Yusupov WB Human 02/22/2018

Summary

• Application: Western Blot
• Sample Tested: C4-2 human prostate cancer cell line,LNCaP human prostate cancer cell line
• Species: Human
• Lot: C2P2E030310

Comments

• Comments: Four siRNAs designed to target the ORF of Importin 7 were transfected into C4-2 human prostate cancer cells (~80% confluence) in a 6-well plate, along with non-target siRNA and transfection reagent-only controls. Cells were lysed with RIPA buffer after 3 days of incubation followed by SDS-PAGE on 10% polyacrylamide gel and transfer to PVDF membrane. Membrane was blocked with 5% milk for 1 hr, washed with 1X TBST, and incubated with 1:1000 dilution of anti-importin 7 primary antibody (NB100-1081) in 3% BSA overnight at 4 degrees Celsius. Membranes were washed with 1X TBST, and incubated with 1:500 dilution of mouse anti-goat secondary antibody in 5% milk for 1 hr at room temperature. Loading control was measured by incubating with 1:10,000 anti-GAPDH-HRP in 1X TBST for 30min.

FAQs for Importin-7 Antibody (NB100-1081). (Showing 1 - 1 of 1 FAQs).

1. I have noticed that you have a rabbit polyclonal anti-human Ipo7. I am searching for a primary mouse/goat ab anti-human Importin7 (Ipo7) that is suitable for IF, WB application. Could you please tell me if you have something that is suitable for me?
   • NBP1-87880 suits all of your needs but is a rabbit host. I am not sure if this will work for you or not. NB100-1081 is our only goat host antibody and it has been validated in Western blot and ELISA. It is expected to work in ICC though, since it is known to recognize the protein in its native form. Please let me know if you have further questions or need further clarification.

Bioinformatics

• Gene Symbol: IPO7
• Entrez:
  • 10527(Human)
• Uniprot:
  • NP_006382.(Human)