Importin-7 Antibody

Images

 
Flow Cytometry: Importin-7 Antibody [NB100-1081] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications Flow, PEP-ELISA, KD
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Importin-7 Antibody Summary

Immunogen
Peptide with sequence C-SSFNFGGPAPGMN corresponding to C-Terminus according to NP_006382.1.
Localization
Nuclear membrane
Predicted Species
Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IPO7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 ug/mL
  • Peptide ELISA Detection limit 1:4000
  • Knockdown Validated
Application Notes
WB: Approx. 120 kDa band observed in A431, Daudi and HeLa lysates (calculated MW of 120 kDa band according to NP_006382.1). An additional band of unknown identity was also consistently observed at 26-28 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Control
Human Brain Whole Tissue Lysate (Adult Whole Normal)
Reviewed Applications
Read 1 Review rated 5
using
NB100-1081 in the following applications:

Publications
Read Publications using NB100-1081.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Importin-7 Antibody

  • FLJ14581
  • Imp7MGC138673
  • importin 7
  • RAN binding protein 7
  • Ran-binding protein 7
  • RanBP7
  • RANBP7importin-7

Background

The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran binding site of importin beta. Similar to importin beta, this protein prevents the activation of Ran's GTPase by RanGAP1 and inhibits nucleotide exchange on RanGTP, and also binds directly to nuclear pore complexes where it competes for binding sites with importin beta and transportin. This protein has a Ran-dependent transport cycle and it can cross the nuclear envelope rapidly and in both directions. At least four importin beta like transport receptors, namely importin beta itself, transportin, RanBP5 and importin 7, directly bind and import ribosomal proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-61832
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP2-24751
Species: Hu, Pm
Applications: WB, Simple Western, ICC/IF, IP
MAB1230
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC, KO
NBP2-22203
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
H00003843-M01
Species: Hu, Mu, Rt, Ca
Applications: WB, ELISA, IHC, IHC-P
NBP1-82417
Species: Rt
Applications: WB
NBP2-45184
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-31413
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, KD
NBP1-85533
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF2039
Species: Hu
Applications: WB, Simple Western, IHC
NBP1-92626
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NB100-93304
Species: Hu, ChHa
Applications: WB, IP
NB600-335
Species: All-NA
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
AF3797
Species: Hu, Mu
Applications: WB, Simple Western, ChIP, ICC
NB400-151
Species: Hu, Mu, Rt
Applications: ICC/IF (-), WB
NB100-79802
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP

Publications for Importin-7 Antibody (NB100-1081)(4)

Review for Importin-7 Antibody (NB100-1081) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-1081:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Importin-7 NB100-1081
Enlarge
5
reviewed by:
Ariel Yusupov
WB Human 02/22/2018
View

Summary

ApplicationWestern Blot
Sample TestedC4-2 human prostate cancer cell line,LNCaP human prostate cancer cell line
SpeciesHuman
LotC2P2E030310

Comments

CommentsFour siRNAs designed to target the ORF of Importin 7 were transfected into C4-2 human prostate cancer cells (~80% confluence) in a 6-well plate, along with non-target siRNA and transfection reagent-only controls. Cells were lysed with RIPA buffer after 3 days of incubation followed by SDS-PAGE on 10% polyacrylamide gel and transfer to PVDF membrane. Membrane was blocked with 5% milk for 1 hr, washed with 1X TBST, and incubated with 1:1000 dilution of anti-importin 7 primary antibody (NB100-1081) in 3% BSA overnight at 4 degrees Celsius. Membranes were washed with 1X TBST, and incubated with 1:500 dilution of mouse anti-goat secondary antibody in 5% milk for 1 hr at room temperature. Loading control was measured by incubating with 1:10,000 anti-GAPDH-HRP in 1X TBST for 30min.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Importin-7 Antibody (NB100-1081). (Showing 1 - 1 of 1 FAQs).

  1. I have noticed that you have a rabbit polyclonal anti-human Ipo7. I am searching for a primary mouse/goat ab anti-human Importin7 (Ipo7) that is suitable for IF, WB application. Could you please tell me if you have something that is suitable for me?
    • NBP1-87880 suits all of your needs but is a rabbit host. I am not sure if this will work for you or not. NB100-1081 is our only goat host antibody and it has been validated in Western blot and ELISA. It is expected to work in ICC though, since it is known to recognize the protein in its native form. Please let me know if you have further questions or need further clarification.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional Importin-7 Products

Bioinformatics Tool for Importin-7 Antibody (NB100-1081)

Discover related pathways, diseases and genes to Importin-7 Antibody (NB100-1081). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Importin-7 Antibody (NB100-1081)

Discover more about diseases related to Importin-7 Antibody (NB100-1081).
 

Pathways for Importin-7 Antibody (NB100-1081)

View related products by pathway.

PTMs for Importin-7 Antibody (NB100-1081)

Learn more about PTMs related to Importin-7 Antibody (NB100-1081).

Blogs on Importin-7

There are no specific blogs for Importin-7, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Ariel Yusupov
02/22/2018
Application: WB
Species: Human

Bioinformatics

Gene Symbol IPO7
Entrez
Uniprot