ILKAP/PP2C delta Antibody

Immunoprecipitation: ILKAP/PP2C delta Antibody [NB100-74606] - ILKAP Antibody [NB100-74606] - Detection of Human ILKAP by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ILKAP antibody NB100-74606 used for IP at 3 mcg/mg lysate. ILKAP was also immunoprecipitated by rabbit anti- ILKAP antibody NB100-74607, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: IP, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

ILKAP/PP2C delta Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 50 and 100 of human integrin-linked kinase-associated serine/threonine phosphatase 2C using the numbering given in entry NP_110395.1 (GeneID 80895).
• Localization: Cytoplasmic
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ILKAP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10-1:500
• Application Notes: This antibody is not recommended for Western Blot.
• Control:

  ILKAP/PP2C delta Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ILKAP/PP2C delta Antibody

• DKFZp434J2031
• EC 3.1.3.16
• FLJ10181
• ILKAP
• integrin-linked kinase-associated serine/threonine phosphatase 2C
• integrin-linked kinase-associated serine/threonine phosphatase
• MGC4846
• PP2C delta
• PP2C-DELTA
• protein phosphatase 2c, delta isozyme

Background

The protein encoded by this gene is a protein serine/threonine phosphatase of the PP2C family. This protein can interact with integrin-linked kinase (ILK/ILK1), a regulator of integrin mediated signaling, and regulate the kinase activity of ILK. Through the interaction with ILK, this protein may selectively affect the signaling process of ILK-mediated glycogen synthase kinase 3 beta (GSK3beta), and thus participate in Wnt signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ILKAP
• Entrez:
  • 80895(Human)
• Uniprot:
  • NP_110395.1(Human)