ILF2 Antibody

Images

 
Western Blot: ILF2 Antibody [NBP1-71902] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified ...read more
Immunohistochemistry: ILF2 Antibody [NBP1-71902] - Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti- NF45 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: ...read more
Immunohistochemistry: ILF2 Antibody [NBP1-71902] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- NF45 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

ILF2 Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 340 and 390 of human Nuclear Factor of Activated T-cells 45 kDa using the numbering given in entry NP_004506.2 (GeneID 3608).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ILF2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 2-10 mg/mg
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ILF2 Antibody

  • interleukin enhancer binding factor 2, 45kD
  • interleukin enhancer binding factor 2, 45kDa
  • interleukin enhancer-binding factor 2
  • MGC8391
  • NF45PRO3063
  • Nuclear factor of activated T-cells 45 kDa
  • nuclear factor of activated T-cells, 45-kDa

Background

Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of the interleukin 2 gene. NFAT binds to a sequence in the interleukin 2 gene enhancer known as the antigen receptor response element 2.In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the smaller of which is the product of thisgene. The encoded protein binds strongly to the 90 kDa protein and stimulates its ability to enhance gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ILF2
Uniprot