Recombinant Mouse IL12A/IL12B His Protein

SDS-Page: Recombinant Mouse IL12A/IL12B Protein [NBP2-61988] - 3ug by SDS-PAGE visualized by coomassie blue stain. Lane 1: reducing condition, Lane 2: non-reducing condition

Bioactivity: Recombinant Mouse IL12A/IL12B Protein [NBP2-61988] - Mouse IL12 stimulates IFN-g secretion of the NK-92 human natural killer cells. The ED50 range is less or equal to 10 ng/ml.

Product Discontinued

Recombinant Mouse IL12A/IL12B His Protein Summary

• Description: A Biologically active recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 23-335 of Mouse IL-12
  
  

  Source:Baculovirus

  Amino Acid Sequence: IL12B(p40);; MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS;; IL12A(p35);; RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSAHHHHHH

• Details of Functionality: Mouse IL12A/IL12B stimulates IFN-g secretion of the NK-92 human natural killer cells. The ED50 range is less or equal to 10 ng/ml.
• Source: Baculovirus
• Protein/Peptide Type: Recombinant Protein
• Gene: IL12A
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1ug of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 35.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% glycerol
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Mouse IL12A/IL12B His Protein

• CLMF p35
• CLMF p40
• CLMF
• CLMF2
• cytotoxic lymphocyte maturation factor 1, p35
• Cytotoxic lymphocyte maturation factor 35 kDa subunit
• Cytotoxic lymphocyte maturation factor 40 kDa subunit
• IL-12, subunit p35
• IL-12A
• IL-12B
• IL35 subunit
• IMD28
• IMD29
• interleukin 12, p35
• interleukin 12, p40
• interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
• interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
• interleukin-12 alpha chain
• interleukin-12 beta chain
• natural killer cell stimulatory factor, 40 kD subunit
• NF cell stimulatory factor chain 1
• NFSK
• NK cell stimulatory factor chain 1
• NK cell stimulatory factor chain 2
• NKSF
• NKSF1
• NKSF2
• p35

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IL12A