IL-36 gamma/IL-1F9 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

IL-36 gamma/IL-1F9 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human IL‑36 gamma /IL‑1F9
  Met1-Asp169
  Accession # Q9NZH8
• Specificity: Detects human IL‑36 gamma /IL‑1F9 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) IL-36 alpha is observed, and less than 1% cross-reactivity with rhIL-1 alpha , rhIL-1 beta , rhIL-18, rhIL-36Ra, rhIL-37, and rhIL-36 beta is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL36G
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-36 gamma/IL-1F9 Antibody [Biotin]

• IL-1 epsilon
• IL-1 H1
• IL-1 Related Protein 2
• IL-1(EPSILON)
• IL1E
• IL-1-epsilon
• IL1F9
• IL-1F9
• IL1H1
• IL-1H1
• IL-1-Related Protein 2
• IL1RP2
• IL-1rp2
• IL36 gamma
• IL-36 gamma
• IL36G
• interleukin 1 family, member 9
• interleukin 1-related protein 2
• Interleukin 36, Gamma
• Interleukin-1 epsilon
• interleukin-1 family member 9
• Interleukin-1 homolog 1
• Interleukin-36 Gamma

Background

Human interleukin 1 family member #9 [IL-1F9; also named IL-1 epsilon  (epsilon) and IL-1H1] is a member of the IL-1 family, which includes IL-1 beta , IL-1 alpha , IL-1ra, IL-18 and IL-1F5 through F10 (1‑4). All family members show a 12 beta -strand, beta -trefoil configuration, and are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). IL-1F9 is synthesized as a 19 kDa, 169 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site (1, 2, 5). The molecule is secreted when transfected into 293-T cells (5). Human to mouse, IL-1F9 has 53% aa sequence identity. Within the family, IL-1F9 shares 30%, 23%, 33%, 57%, 35%, 45%, and 32% aa sequence identity with IL-1 ra, IL-1 beta , IL-1F5, F6, F7, F8, and F10, respectively. Cells reported to express IL-1F9 include Langerhans cells, keratinocytes/stratified squamous epithelium, plus Chief cells and Parietal cells of the stomach (4, 5). The receptor for IL‑1F9 is reported to be a combination of IL-1 Rrp2 and IL-1 RAcP (6). Recombinant IL-1F9, along with IL-1F8 and IL-1F6, has been shown to activate the pathway involving NF-kappa B and MAPK in an IL-1 Rrp2 dependent manner.

1. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
2. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
3. Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
4. Dunn, E. et al. (2001) Trends Immunol. 22:533.
5. Debets, R. et al. (2001) J. Immunol. 167:1440.
6. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-36 gamma/IL-1F9 Antibody (BAF2320)(6)

Publications using BAF2320

• Todorovic, McDonald HA, Hoover P et al. Cytokine induced 3-D organotypic psoriasis skin model demonstrates distinct roles for NF-kappa B and JAK pathways in disease pathophysiology Experimental dermatology 2/25/2022 [PMID: 35213752]
• Thomas Macleod, Joseph S. Ainscough, Christina Hesse, Sebastian Konzok, Armin Braun, Anna-Lena Buhl et al. The Proinflammatory Cytokine IL-36 gamma Is a Global Discriminator of Harmless Microbes and Invasive Pathogens within Epithelial Tissues Cell Reports 2020-12-15 [PMID: 33326792]
• JS Ainscough, T Macleod, D McGonagle, R Brakefield, JM Baron, A Alase, M Wittmann, M Stacey Cathepsin S is the major activator of the psoriasis-associated proinflammatory cytokine IL-36? Proc. Natl. Acad. Sci. U.S.A, 2017-03-13;114(13):E2748-E2757. 2017-03-13 [PMID: 28289191] (Western Blot, Human)
• Na Li, Kenshi Yamasaki, Rumiko Saito, Sawako Fukushi-Takahashi, Ryoko Shimada-Omori, Masayuki Asano et al. Alarmin Function of Cathelicidin Antimicrobial Peptide LL37 through IL-36 gamma Induction in Human Epidermal Keratinocytes The Journal of Immunology 2014-11-15 [PMID: 25305315]
• Carrier Y, Ma HL, Ramon HE, Napierata L, Small C, O'Toole M, Young DA, Fouser LA, Nickerson-Nutter C, Collins M, Dunussi-Joannopoulos K, Medley QG Inter-regulation of Th17 cytokines and the IL-36 cytokines in vitro and in vivo: implications in psoriasis pathogenesis. J. Invest. Dermatol., 2011-09-01;131(12):2428-37. 2011-09-01 [PMID: 21881584] (Western Blot, Human)
• Johnston A, Xing X, Guzman AM, Riblett M, Loyd CM, Ward NL, Wohn C, Prens EP, Wang F, Maier LE, Kang S, Voorhees JJ, Elder JT, Gudjonsson JE IL-1F5, -F6, -F8, and -F9: A Novel IL-1 Family Signaling System That Is Active in Psoriasis and Promotes Keratinocyte Antimicrobial Peptide Expression. J. Immunol., 2011-01-17;186(4):2613-22. 2011-01-17 [PMID: 21242515] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: IL36G
• Uniprot:
  • Q9NZH8()