IL-36 gamma/IL-1F9 Antibody [Unconjugated]

Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 20 ng/mL Recombinant Human IFN-gamma (Catalog # 285-IF) and 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA) for 6 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL-36 gamma/IL-1F9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2320) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-36 gamma/IL-1F9 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-36 gamma/IL-1F9 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human IL‑36 gamma /IL‑1F9 (R&D Systems, Catalog # 2320-IL)
  Met1-Asp169
  Accession # Q9NZH8
• Specificity: Detects human IL‑36 gamma /IL‑1F9 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) IL-36 alpha is observed, approximately 5% cross-reactivity with rhIL-37 is observed, and less than 1% cross-reactivity with rhIL-1 alpha , rhIL-1 beta , rhIL‑36Ra, and rhIL‑36 beta is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL36G
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction 10 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-36 gamma/IL-1F9 Antibody [Unconjugated]

• IL-1 epsilon
• IL-1 H1
• IL-1 Related Protein 2
• IL-1(EPSILON)
• IL1E
• IL-1-epsilon
• IL1F9
• IL-1F9
• IL1H1
• IL-1H1
• IL-1-Related Protein 2
• IL1RP2
• IL-1rp2
• IL36 gamma
• IL-36 gamma
• IL36G
• interleukin 1 family, member 9
• interleukin 1-related protein 2
• Interleukin 36, Gamma
• Interleukin-1 epsilon
• interleukin-1 family member 9
• Interleukin-1 homolog 1
• Interleukin-36 Gamma

Background

Human interleukin 1 family member #9 [IL-1F9; also named Interleukin-36 gamma, IL36G, IL-1 epsilon  (epsilon) and IL-1H1] is a member of the IL-1 family, which includes IL-1 beta , IL-1 alpha , IL-1ra, IL-18 and IL-1F5 through F10 (1‑4). All family members show a 12 beta -strand, beta -trefoil configuration, and are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). IL-1F9 is synthesized as a 19 kDa, 169 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site (1, 2, 5). The molecule is secreted when transfected into 293-T cells (5). Human to mouse, IL-1F9 has 53% aa sequence identity. Within the family, IL-1F9 shares 30%, 23%, 33%, 57%, 35%, 45%, and 32% aa sequence identity with IL-1 ra, IL-1 beta , IL-1F5, F6, F7, F8, and F10, respectively. Cells reported to express IL-1F9 include Langerhans cells, keratinocytes/stratified squamous epithelium, plus Chief cells and Parietal cells of the stomach (4, 5). The receptor for IL‑1F9 is reported to be a combination of IL-1 Rrp2 and IL-1 RAcP (6). Recombinant IL-1F9, along with IL-1F8 and IL-1F6, has been shown to activate the pathway involving NF-kappa B and MAPK in an IL-1 Rrp2 dependent manner.

1. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
2. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
3. Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
4. Dunn, E. et al. (2001) Trends Immunol. 22:533.
5. Debets, R. et al. (2001) J. Immunol. 167:1440.
6. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-36 gamma/IL-1F9 Antibody (AF2320)(6)

Publications using AF2320

• Jonathan R. Baker, Peter S. Fenwick, Carolin K. Koss, Harriet B. Owles, Sarah L. Elkin, Jay Fine et al. IL-36 receptor agonist and antagonist imbalance drives neutrophilic inflammation in COPD JCI Insight 2022-08-08 [PMID: 35763349]
• JM Murrieta-C, RN Gutiérrez-, HM El-Shorafa, T Groten, S Rodríguez-, ME Cancino-Di, JC Cancino-Di, RR Favaro, UR Markert, DM Morales-Pr Role of IL-36 Cytokines in the Regulation of Angiogenesis Potential of Trophoblast Cells International Journal of Molecular Sciences, 2020-12-30;22(1):. 2020-12-30 [PMID: 33396613] (Western Blot, Human)
• Takashi K. Satoh, Mark Mellett, Barbara Meier-Schiesser, Gabriele Fenini, Atsushi Otsuka, Hans-Dietmar Beer et al. IL-36 gamma drives skin toxicity induced by EGFR/MEK inhibition and commensal Cutibacterium acnes Journal of Clinical Investigation 2020-02-04 [PMID: 31805013]
• Scheiermann P, Bachmann M, Hardle L, Pleli T, Piiper A, Zwissler B, Pfeilschifter J, Muhl H Application of IL-36 receptor antagonist weakens CCL20 expression and impairs recovery in the late phase of murine acetaminophen-induced liver injury. Sci Rep, 2015-02-17;5(0):8521. 2015-02-17 [PMID: 25687687] (Western Blot, Human)
• Bachmann M, Scheiermann P, Hardle L, Pfeilschifter J, Muhl H IL-36gamma/IL-1F9, an innate T-bet target in myeloid cells. J Biol Chem, 2012-10-24;287(50):41684-96. 2012-10-24 [PMID: 23095752] (Western Blot, Human)
• Lian LH, Milora KA, Manupipatpong KK, Jensen LE The double-stranded RNA analogue polyinosinic-polycytidylic acid induces keratinocyte pyroptosis and release of IL-36 gamma. J. Invest. Dermatol., 2012-02-09;132(5):1346-53. 2012-02-09 [PMID: 22318382] (ELISA Development, Western Blot, Human)

Bioinformatics

• Gene Symbol: IL36G
• Uniprot:
  • Q9NZH8()