IL-36 beta/IL-1F8 Antibody [Alexa Fluor® 647]

• Reactivity: Mu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 647

IL-36 beta/IL-1F8 Antibody [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant mouse IL‑36β/IL‑1F8
  Met1-Lys183
  Accession # Q9D6Z6
• Specificity: Detects mouse IL-36 beta /IL-1F8 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) IL-1F8 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL36B
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-36 beta/IL-1F8 Antibody [Alexa Fluor® 647]

• family of interleukin 1-eta
• FIL1 eta
• FIL1
• FIL1-(ETA)
• FIL1H
• FILI-(ETA)
• IL-1 eta
• IL1-ETA
• IL1F8 (Canonical product IL-1F8a)
• IL-1F8 (FIL1-eta)
• IL1F8
• IL-1F8
• IL1H2
• IL-1H2
• IL36 beta
• IL-36 beta
• IL36B
• interleukin 1 family, member 8 (eta)
• interleukin 1, eta
• Interleukin 36, Beta
• Interleukin-1 eta
• interleukin-1 family member 8
• Interleukin-1 homolog 2
• Interleukin-1 Superfamily e
• Interleukin-36 Beta

Background

Mouse interleukin-36 beta [IL-36 beta ; also named IL-1F8, interleukin 1 family member 8, FIL-1 eta (eta) and IL-1H2] is a member of the IL-1 family of proteins (1-3). IL-1 family members include IL-1 beta , IL-1 alpha , IL-1ra, IL-18 and IL-1F5 through F10 (4). All family members show a 12 beta -stranded beta -trefoil configuration, and are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). Although two alternatively spliced transcript variants for human IL-1F8 have been described, to date, only one mouse IL-1F8 isoform is known (3). Mouse IL-1F8 is synthesized as a 183 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site(s) (1, 2). It is likely that mouse IL-1F8, similarly to its human homologue, is actively secreted (1). Mouse IL-1F8 shares 61% and 74% aa identity with human IL-1F8 isoform 2 and rat IL-1F8, respectively. Within the IL-1 family, IL-1F8 shares 27% sequence with 33%, 32%, 35%, 39% and 28% aa sequence identity with IL-1 ra, IL-1 beta , IL-1F5, F6, F9 and F10, respectively. Cells reported to express IL-1F8 include resting and activated monocytes and B cells (1, 4). The receptor for IL-1F8 is reported to be a combination of IL-1 Rrp2 and IL-1 RAcP (5). Recombinant IL-1F8, along with IL-1F6 and IL-1F9, has been shown to activate the pathway involving NF-kappa B and MAPK in an IL-1Rrp2 dependent manner.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL36B
• Uniprot:
  • Q9D6Z6()