IL-36 alpha/IL-1F6 Antibody [Unconjugated]

Western blot shows lysates of human tonsil tissue, HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-36a/IL-1F6. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL-36a/IL-1F6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1078) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-36a/IL-1F6 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IL‑36 alpha /IL‑1F6 was detected in perfusion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL‑36 alpha /IL‑1F6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1078) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-36 alpha/IL-1F6 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human IL‑36 alpha /IL‑1F6
  Met1-Phe158
  Accession # Q9UHA7
• Specificity: Detects human IL‑36 alpha /IL‑1F6 in direct ELISAs and Western blots. In Western blots, less than 2% cross‑reactivity with recombinant human (rh) rhIL-36 beta , rhIL-36 gamma , rhIL-1 alpha , rhIL-1 beta and rhIL-18 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL36A
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-36 alpha/IL-1F6 Antibody [Unconjugated]

• FIL1 epsilon
• FIL1
• FIL1(EPSILON)
• FIL1E
• IL-1 epsilon
• IL1(EPSILON)
• IL1E
• IL-1E
• IL-1F6 (FIL-1-epsilon)
• IL1F6
• IL-1F6
• IL36 alpha
• IL-36 alpha
• IL36A
• interleukin 1 family, member 6 (epsilon)
• interleukin 1, epsilon
• Interleukin 36, Alpha
• Interleukin-1 epsilon
• interleukin-1 family member 6
• Interleukin-36 Alpha

Background

Human interleukin 1 family member #6 [IL-1F6; also Interleukin-36 alpha, IL36A, FIL-1 epsilon  (epsilon)] is a member of the IL-1 family of proteins (1‑3). IL-1 family members include IL-1 beta , IL-1 alpha , IL‑1ra, IL-18 and IL-1F5 through F10 (4). All family members show a 12 beta -strand, beta -trefoil configuration, and all family members are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). IL-1F6 is synthesized as a 158 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site(s) (1‑3). It appears to be actively secreted (1). When found in cell lysate, it presents as an 18 kDa monomer (2). Human to mouse, full length IL-1F6 has 54% aa identity. Within the family, IL-1F6 is 30% aa identical to IL-1ra, and 27%, 31%, 36%, 46%, 57% and 28% aa identical to IL-1 beta , IL-1F5, F7, F8, F9 and F10, respectively. Cells reported to express IL-1F6 include monocytes, B cells and T cells (1, 4). Notably, IL-1F6 is the only novel IL-1 family member found to be expressed on T-cells. The receptor for IL-1F6 is reported to be a combination of IL-1 Rrp2 and IL-1 RAcP (5). Recombinant IL‑1F6, along with IL-1F8 and IL-1F9, has been shown to act as an agonist by activating the pathway involving NF-kappa B and MAPK in an IL‑1 Rrp2 dependent manner. This suggests that IL-1F6 may signal in a similar fashion to IL-1 and IL-18 in having a binding receptor which upon ligation, recruits a second receptor as a signaling component, forming an active heterodimeric receptor complex.

1. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
2. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
3. Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
4. Dunn, E. et al. (2001) Trends Immunol. 22:533.
5. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-36 alpha/IL-1F6 Antibody (AF1078)(5)

Publications using AF1078

• Zhan, ZY;Zhang, ZH;Sun, RH;Wu, YL;Nan, JX;Lian, LH; A therapeutic strategy of parthenolide in improving imiquimod-induced psoriasis-like skin inflammation targeting IL-36/NETs through skin transdermal therapeutic system International immunopharmacology 2024-03-09 [PMID: 38461633] (Western Blot, Human)
• JM Murrieta-C, RN Gutiérrez-, HM El-Shorafa, T Groten, S Rodríguez-, ME Cancino-Di, JC Cancino-Di, RR Favaro, UR Markert, DM Morales-Pr Role of IL-36 Cytokines in the Regulation of Angiogenesis Potential of Trophoblast Cells International Journal of Molecular Sciences, 2020-12-30;22(1):. 2020-12-30 [PMID: 33396613] (Western Blot, Human)
• V Schmitt, M Hahn, V Kästele, O Wagner, M Wiendl, A Derer, A Taddeo, S Hahne, A Radbruch, HM Jäck, W Schuh, D Mielenz, S Gay, G Schett, AJ Hueber, S Frey Interleukin-36 receptor mediates the crosstalk between plasma cells and synovial fibroblasts Eur. J. Immunol., 2017-09-22;0(0):. 2017-09-22 [PMID: 28857172] (Western Blot, Human)
• Johnston Andrew, Xing Xianying, Wolterink Liza et al. IL-1 and IL-36 are dominant cytokines in generalized pustular psoriasis. The Journal of Allergy and Clinical Immunology 1/1/2017 [PMID: 28043870]
• Johnston A, Xing X, Guzman AM, Riblett M, Loyd CM, Ward NL, Wohn C, Prens EP, Wang F, Maier LE, Kang S, Voorhees JJ, Elder JT, Gudjonsson JE IL-1F5, -F6, -F8, and -F9: A Novel IL-1 Family Signaling System That Is Active in Psoriasis and Promotes Keratinocyte Antimicrobial Peptide Expression. J. Immunol., 2011-01-17;186(4):2613-22. 2011-01-17 [PMID: 21242515] (IHC-Fr, Human)

Bioinformatics

• Gene Symbol: IL36A
• Uniprot:
  • Q9UHA7()