IL-36 alpha/IL-1F6 Antibody (162122) [Unconjugated]

Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-36a/IL-1F6. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL-36a/IL-1F6 Monoclonal Antibody (Catalog # MAB1078) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-36a/IL-1F6 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 162122
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-36 alpha/IL-1F6 Antibody (162122) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human IL‑36 alpha /IL‑1F6
  Met1-Phe158
  Accession # Q9UHA7
• Specificity: Detects human IL‑36 alpha /IL‑1F6 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-1 alpha , rhIL-1 beta , rhIL-1 receptor antagonist, rhIL-18, rhIL-36Ra, rhIL-36 beta or recombinant mouse IL-36 alpha is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL36A
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-36 alpha/IL-1F6 Antibody (162122) [Unconjugated]

• FIL1 epsilon
• FIL1
• FIL1(EPSILON)
• FIL1E
• IL-1 epsilon
• IL1(EPSILON)
• IL1E
• IL-1E
• IL-1F6 (FIL-1-epsilon)
• IL1F6
• IL-1F6
• IL36 alpha
• IL-36 alpha
• IL36A
• interleukin 1 family, member 6 (epsilon)
• interleukin 1, epsilon
• Interleukin 36, Alpha
• Interleukin-1 epsilon
• interleukin-1 family member 6
• Interleukin-36 Alpha

Background

Human interleukin 1 family member #6 [IL-1F6; also IL-36 alpha and FIL-1 epsilon (epsilon)] is a member of the IL-1 family of proteins (1‑3, 6). IL-1 family members include IL-1 beta , IL-1 alpha , IL‑1ra, IL-18 and IL-1F5 through F10 (4). All family members show a 12 beta -strand, beta -trefoil configuration, and all family members are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). IL-36 alpha is synthesized as a 158 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site(s) (1‑3). It appears to be actively secreted (1). When found in cell lysate, it presents as an 18 kDa monomer (2). Human to mouse, full length IL-36 alpha has 54% aa identity. Within the family, IL-36 alpha is 30% aa identical to IL-1ra, and 27%, 31%, 36%, 46%, 57% and 28% aa identical to IL-1 beta , IL-36Ra, IL-1F7, IL-36 beta , IL-36 gamma , and IL-1F10, respectively. Cells reported to express IL-36 alpha include monocytes, B cells and T cells (1, 4). Notably, IL-36 alpha is the only novel IL-1 family member found to be expressed on T-cells. The receptor for IL-36 alpha is reported to be a combination of IL-1 Rrp2 and IL-1 RAcP (5). Recombinant IL-36 alpha , along with IL-36 beta and IL-36 gamma , has been shown to act as an agonist by activating the pathway involving NF-kappa B and MAPK in an IL-1 Rrp2 dependent manner. This suggests that IL-36 alpha may signal in a similar fashion to IL-1 and IL-18 in having a binding receptor which upon ligation, recruits a second receptor as a signaling component, forming an active heterodimeric receptor complex.

1. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
2. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
3. Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
4. Dunn, E. et al. (2001) Trends Immunol. 22:533.
5. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
6. Dinarello, C. et al. (2010) Nat. Immunol. 11:973.
   

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-36 alpha/IL-1F6 Antibody (MAB1078)(1)

Publication using MAB1078

• Southcombe J, Redman C, Sargent I, Granne I Interleukin-1 family cytokines and their regulatory proteins in normal pregnancy and pre-eclampsia. Clin Exp Immunol, 2015-06-29;181(3):480-90. 2015-06-29 [PMID: 25693732] (Western Blot, Human)

Bioinformatics

• Gene Symbol: IL36A
• Uniprot:
  • Q9UHA7()