IL-36 alpha/IL-1F6 Antibody (162122) [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: WB
• Clone: 162122
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

IL-36 alpha/IL-1F6 Antibody (162122) [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human IL‑36α/IL‑1F6
  Met1-Phe158
  Accession # Q9UHA7
• Specificity: Detects human IL‑36 alpha /IL‑1F6 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-1 alpha , rhIL-1 beta , rhIL-1 receptor antagonist, rhIL-18, rhIL-36Ra, rhIL-36 beta or recombinant mouse IL-36 alpha is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-36 alpha/IL-1F6 Antibody (162122) [Alexa Fluor® 594]

• FIL1 epsilon
• FIL1
• FIL1(EPSILON)
• FIL1E
• IL-1 epsilon
• IL1(EPSILON)
• IL1E
• IL-1E
• IL-1F6 (FIL-1-epsilon)
• IL1F6
• IL-1F6
• IL36 alpha
• IL-36 alpha
• IL36A
• interleukin 1 family, member 6 (epsilon)
• interleukin 1, epsilon
• Interleukin 36, Alpha
• Interleukin-1 epsilon
• interleukin-1 family member 6
• Interleukin-36 Alpha

Background

Human interleukin 1 family member #6 [IL-1F6; also IL-36 alpha and FIL-1 epsilon (epsilon)] is a member of the IL-1 family of proteins (1‑3, 6). IL-1 family members include IL-1 beta , IL-1 alpha , IL‑1ra, IL-18 and IL-1F5 through F10 (4). All family members show a 12 beta -strand, beta -trefoil configuration, and all family members are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). IL-36 alpha is synthesized as a 158 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site(s) (1‑3). It appears to be actively secreted (1). When found in cell lysate, it presents as an 18 kDa monomer (2). Human to mouse, full length IL-36 alpha has 54% aa identity. Within the family, IL-36 alpha is 30% aa identical to IL-1ra, and 27%, 31%, 36%, 46%, 57% and 28% aa identical to IL-1 beta , IL-36Ra, IL-1F7, IL-36 beta , IL-36 gamma , and IL-1F10, respectively. Cells reported to express IL-36 alpha include monocytes, B cells and T cells (1, 4). Notably, IL-36 alpha is the only novel IL-1 family member found to be expressed on T-cells. The receptor for IL-36 alpha is reported to be a combination of IL-1 Rrp2 and IL-1 RAcP (5). Recombinant IL-36 alpha , along with IL-36 beta and IL-36 gamma , has been shown to act as an agonist by activating the pathway involving NF-kappa B and MAPK in an IL-1 Rrp2 dependent manner. This suggests that IL-36 alpha may signal in a similar fashion to IL-1 and IL-18 in having a binding receptor which upon ligation, recruits a second receptor as a signaling component, forming an active heterodimeric receptor complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.