Human IL-3 ELISA Kit (Colorimetric)

ELISA: Human IL-3 ELISA Kit (Colorimetric) [NBP2-50063] - IL-3 ELISA Kit [NBP2-50063] - Typical Standard Curve: These standard curves are for demonstration only. A standard curve must be run with each assay.

Product Discontinued

Human IL-3 ELISA Kit (Colorimetric) Summary

• Description: This kit is a sandwich ELISA assay for the quantitative measurement of human IL-3 in serum, plasma, tissue homogenates and cell culture supernatants. The density of color is proportional to the amount of IL-3 captured from the samples.
• Standard Curve Range: 15.6 - 1000 pg/mL
• Sensitivity: < 9.4 pg/mL
• Assay Type: Sandwich ELISA
• Sample Volume: 0.1 mL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL3

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Detection Method: Absorbance (450 nm). Quantitative measurement of IL-3 in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Alternate Names for Human IL-3 ELISA Kit (Colorimetric)

• Hematopoietic growth factor
• IL3
• IL-3
• IL-3MGC79398
• interleukin 3 (colony-stimulating factor, multiple)
• interleukin-3
• Mast cell growth factor
• mast-cell growth factor
• MCGF
• MCGFMGC79399
• MULTI-CSF
• multilineage-colony-stimulating factor
• Multipotential colony-stimulating factor
• P-cell stimulating factor
• P-cell-stimulating factor

Background

Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor, P-cell stimulating factor, burst promoting activity, multi-colony stimulating factor, thy-1 inducing factor and WEHI-3 growth factor. In addition to activated T cells, other cell types such as human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes can also produce IL-3. At the amino acid sequence level, mature human and murine IL-3 share only 29% sequence identity. Consistent with this lack of homology, IL-3 activity is highly species-specific and human IL-3 does not show activity on murine cells. IL-3 exerts its biological activities through binding to specific cell surface receptors. The high affinity receptor responsible for IL-3 signaling is composed of at least two subunits, an IL-3 specific alpha chain which binds IL-3 with low affinity and a common beta chain that is shared by the IL-5 and GM-CSF high-affinity receptors. Although the beta chain itself does not bind IL-3, it confers high-affinity IL-3 binding in the presence of the alpha chain. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils and various myeloid leukemic cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for IL-3 ELISA Kit (NBP2-50063). (Showing 1 - 1 of 1 FAQ).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: IL3