IL-3 Antibody

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Recombinant Mouse IL‑3 (Catalog # 403-ML) stimulates proliferation in the M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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IL-3 Antibody Summary

Immunogen
E. coli-derived recombinant mouse IL-3
Asp33-Cys166
Accession # P01586
Specificity
Detects mouse IL-3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human IL-3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL3
Purity
Immunogen affinity purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Neutralization 0.01-0.03 ug/mL
Reviewed Applications
Read 1 Review rated 3
using
AF-403-NA in the following applications:

Publications
Read Publications using
AF-403-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-3 Antibody

  • Hematopoietic growth factor
  • IL3
  • IL-3
  • IL-3MGC79398
  • interleukin 3 (colony-stimulating factor, multiple)
  • interleukin-3
  • Mast cell growth factor
  • mast-cell growth factor
  • MCGF
  • MCGFMGC79399
  • MULTI-CSF
  • multilineage-colony-stimulating factor
  • Multipotential colony-stimulating factor
  • P-cell stimulating factor
  • P-cell-stimulating factor

Background

Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor P-cell stimulating factor, burst promoting activity, multi-colony stimulating factor, thy-1 inducing factor and WEHI-3 growth factor. In addition to activated T cells, other cell types such as human thymic epithelial cells, activated mouse mast cells, mouse keratinocytes and neurons/astrocytes can also produce IL-3. At the amino acid sequence level, mature human and mouse IL-3 share only 29% sequence identity. Consistent with this lack of homology, IL-3 activity is highly species-specific and human IL-3 does not show activity on mouse cells.

IL-3 exerts its biological activities through binding to specific cell surface receptors. The high affinity receptor responsible for IL-3 signaling is composed of alpha and beta subunits. The IL-3R alpha is a member of the cytokine receptor super family and binds IL-3 with low affinity. Two distinct beta subunits, AIC2A ( beta IL-3) and AIC2B ( beta c) are present in mouse cells. beta IL-3 also binds IL-3 with low affinity and forms a high affinity receptor with the alpha subunit. The beta c subunits does not bind any cytokine but forms functional high affinity receptors with the alpha subunit of the IL-3, IL-5 and GM-CSF receptors. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils, and various myeloid leukemic cells.

  1. Yokota, T. et al. (1984) Proc. Natl. Acad. Sci. USA 81:1070.
  2. Fung, M.C. et al. (1984) Nature 307:233.
  3. Miyatake, S. et al. (1985) Proc. Natl. Acad. Sci. USA 82:316.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-3 Antibody (AF-403-NA)(3)

Review for IL-3 Antibody (AF-403-NA) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF-403-NA:
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Simple Western
(1)
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Simple Western IL-3 AF-403-NA
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3
reviewed by:
Anonymous
Simple Western Mouse 06/04/2019
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Summary

ApplicationSimple Western
Sample TestedMouse Spleen
SpeciesMouse
LotAAH0110121

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for IL-3 Antibody (AF-403-NA) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF403

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Bioinformatics Tool for IL-3 Antibody (AF-403-NA)

Discover related pathways, diseases and genes to IL-3 Antibody (AF-403-NA). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for IL-3 Antibody (AF-403-NA)

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Recent Reviews

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Anonymous
06/04/2019
Application: Simple Western
Species: Mouse

Bioinformatics

Gene Symbol IL3
Uniprot