Recombinant Rat IL-1ra/IL-1F3/IL1RN Protein

SDS-Page: Rat IL-1ra/IL-1F3/IL1RN Protein [NBP2-35225]

• Reactivity: Rt
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Rat IL-1ra/IL-1F3/IL1RN Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 152 amino acids corresponding to IL-1ra/IL-1F3/IL1RN Source: E. coli

  Uniprot ID: P25086

  Amino Acid Sequence: HPAGKRPCKM QAFRIWDTNQ KTFYLRNNQL IAGYLQGPNT KLEEKIDMVP IDFRNVFLGI HGGKLCLSCV KSGDDTKLQL EEVNITDLNK NKEEDKRFTF IRSETGPTTS FESLACPGWF LCTTLEADHP VSLTNTPKEP CTVTKFYFQE DQ

• Details of Functionality: IL-1ra/IL-1F3/IL1RN protein is fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1 alpha-dependent proliferation of murine D10S cells is less than 150 ng/ml, corresponding to a specific activity of > 6.7 x 10^3 IU/mg in the presence of 50 pg/ml IL-1ra/IL-1F3/IL1RN
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IL1RN
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of IL-1ra/IL-1F3/IL1RN as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 17.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Rat IL-1ra/IL-1F3/IL1RN Protein

• DIRA
• ICIL-1ra
• IL1F3
• IL-1F3
• IL1ra
• IL-1ra
• IL-1ra3
• IL1RN
• IL-1RN
• interleukin 1 receptor antagonist
• IRAP
• MVCD4

Background

IL-1RA was initially called the IL-1 inhibitor which is encoded by the IL1RN gene and it is a member of the interleukin 1 cytokine family. IL-1RA is secreted by various types of cells including immune cells, epithelial cells, and adipocytes. IL-RA has functions of inhibiting the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. IL-1RA is also used in the treatment of rheumatoid arthritis, an autoimmune disease in which IL- 1 plays a key role. The rat IL-1RA is a single non-glycosylated polypeptide chain containing 152 amino acids and it has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Rat IL-1RA shares 89 % and 73 % a.a. sequence homology with murine and human IL-1RA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IL1RN