IL-1ra/IL-1F3/IL1RN Antibody [Unconjugated]

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Recombinant Mouse IL‑1ra/IL‑1F3 (Catalog # 480‑RM) inhibits Recombinant Human IL‑1 alpha /IL‑1F1 (Catalog # 200‑LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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IL-1ra/IL-1F3/IL1RN Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse IL‑1ra/IL-1F3
Specificity
Detects mouse IL-1ra/IL-1F3 in ELISAs and Western blots. In sandwich ELISAs, approximately 45% cross-reactivity with recombinant rat IL‑1ra/IL‑1F3 and less than 1% cross-reactivity with recombinant human IL‑1ra/IL‑1F3, recombinant porcine IL‑1ra/IL‑1F3, and recombinant equine IL‑1ra/IL‑1F3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL1RN
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Neutralization 1-5 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture:Mouse IL-1ra/IL-1F3 Antibody (Catalog # AF-480-NA)
ELISA Detection: Mouse IL-1ra/IL-1F3 Biotinylated Antibody (Catalog # BAF480)
Standard: Recombinant Mouse IL-1ra/IL-1F3 (Catalog # 480-RM)
Publications
Read Publications using
AF-480-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1ra/IL-1F3/IL1RN Antibody [Unconjugated]

  • DIRA
  • ICIL-1ra
  • IL1F3
  • IL-1F3
  • IL1ra
  • IL-1ra
  • IL-1ra3
  • IL1RN
  • IL-1RN
  • interleukin 1 receptor antagonist
  • IRAP
  • MVCD4

Background

IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The naturally-occurring, fully glycosylated form has an apparent molecular weight of about 25,000 Daltons. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells. In mouse, IL-1 RII does not bind IL-1ra. The recombinant, non-glycosylated form of IL-1ra blocks binding of IL-1 to its receptor equally as well as the naturally-occurring, glycosylated form. The IL-1ra has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Pre-clinical and clinical studies were done to test possible therapeutic applications for IL-1ra in the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-1ra/IL-1F3/IL1RN Antibody (AF-480-NA)(8)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 2 applications: ELISA Development, Neutralization.


Filter By Application
ELISA Development
(3)
Neutralization
(4)
All Applications
Filter By Species
Mouse
(6)
Rat
(2)
All Species
Showing Publications 1 - 8 of 8.
Publications using AF-480-NA Applications Species
X Lu, JW Horner, E Paul, X Shang, P Troncoso, P Deng, S Jiang, Q Chang, DJ Spring, P Sharma, JA Zebala, DY Maeda, YA Wang, RA DePinho Effective combinatorial immunotherapy for castration-resistant prostate cancer Nature, 2017;543(7647):728-732. 2017 [PMID: 28321130] (Neutralization, Mouse) Neutralization Mouse
Correction: Yersinia pestis Activates Both IL-1beta and IL-1 Receptor Antagonist to Modulate Lung Inflammation during Pneumonic Plague. PLoS Pathog, 2015;11(5):e1004921. 2015 [PMID: 25970482] (Mouse) Mouse
Yamato M, Tamura Y, Eguchi A, Kume S, Miyashige Y, Nakano M, Watanabe Y, Kataoka Y Brain interleukin-1beta and the intrinsic receptor antagonist control peripheral Toll-like receptor 3-mediated suppression of spontaneous activity in rats. PLoS ONE, 2014;9(3):e90950. 2014 [PMID: 24621600] (Neutralization, Rat) Neutralization Rat
Osuchowski MF, Connett J, Welch K, Granger J, Remick DG Stratification is the key: inflammatory biomarkers accurately direct immunomodulatory therapy in experimental sepsis. Crit. Care Med., 2009;37(5):1567-73. 2009 [PMID: 19325479] (ELISA Development, Mouse) ELISA Development Mouse
Ortiz LA, Dutreil M, Fattman C, Pandey AC, Torres G, Go K, Phinney DG Interleukin 1 receptor antagonist mediates the antiinflammatory and antifibrotic effect of mesenchymal stem cells during lung injury. Proc. Natl. Acad. Sci. U.S.A., 2007;104(26):11002-7. 2007 [PMID: 17569781] (Neutralization, Mouse) Neutralization Mouse
Osuchowski MF, Welch K, Siddiqui J, Remick DG Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality. J. Immunol., 2006;177(3):1967-74. 2006 [PMID: 16849510] (ELISA Development, Mouse) ELISA Development Mouse
Osuchowski MF, Siddiqui J, Copeland S, Remick DG Sequential ELISA to profile multiple cytokines from small volumes. J. Immunol. Methods, 2005;302(1):172-81. 2005 [PMID: 16023134] (ELISA Development, Mouse) ELISA Development Mouse
Park EM, Cho BP, Volpe BT, Cruz MO, Joh TH, Cho S Ibuprofen protects ischemia-induced neuronal injury via up-regulating interleukin-1 receptor antagonist expression. Neuroscience, 2005;132(3):625-31. 2005 [PMID: 15837124] (Neutralization, Rat) Neutralization Rat

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF480

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Bioinformatics

Gene Symbol IL1RN