IL-1ra/IL-1F3/IL1RN Antibody (40007) [Unconjugated]

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 40007
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

IL-1ra/IL-1F3/IL1RN Antibody (40007) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse IL-1ra
  Arg27-Gln178
  Accession # P25085
• Specificity: Detects human and mouse IL-1ra in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: IL1RN
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1ra/IL-1F3/IL1RN Antibody (40007) [Unconjugated]

• DIRA
• ICIL-1ra
• IL1F3
• IL-1F3
• IL1ra
• IL-1ra
• IL-1ra3
• IL1RN
• IL-1RN
• interleukin 1 receptor antagonist
• IRAP
• MVCD4

Background

IL-1ra (IL-1 receptor antagonist) is a widely expressed acute phase protein that serves to dampen inflammation. IL-1ra binds to both IL-1 RI and IL-1 RII. It competitively inhibits IL-1 (alpha or beta) binding to IL-1 RI and does not trigger recruitment of the accessory molecule IL-1 R AcP or activation of IL-1 RI signaling. IL-1ra blocks the proinflammatory actions of IL-1 including Prostaglandin E2 and IL-6 release from endothelial cells, fever generation, thrombocytosis, the production of hepatic acute phase proteins, and neutrophil expansion and infiltration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-1ra/IL-1F3/IL1RN Antibody (MAB480)(3)

Publications using MAB480

• BH Clausen, M Wirenfeldt, SS Høgedal, LH Frich, HH Nielsen, HD Schrøder, K Østergaard, B Finsen, BW Kristensen, KL Lambertsen Characterization of the TNF and IL-1 systems in human brain and blood after ischemic stroke Acta Neuropathol Commun, 2020-06-05;8(1):81. 2020-06-05 [PMID: 32503645] (IHC, Human)
• Murtuza B, Suzuki K, Bou-Gharios G, Beauchamp JR, Smolenski RT, Partridge TA, Yacoub MH Transplantation of skeletal myoblasts secreting an IL-1 inhibitor modulates adverse remodeling in infarcted murine myocardium. Proc. Natl. Acad. Sci. U.S.A., 2004-03-12;101(12):4216-21. 2004-03-12 [PMID: 15020774] (IHC-P, Mouse)
• Arjen B Blom, Peter L van Lent, Hanneke van Vuuren, Astrid E Holthuysen, Cor Jacobs, Leo B van de Putte et al. FcgammaR expression on macrophages is related to severity and chronicity of synovial inflammation and cartilage destruction during experimental immune-complex-mediated arthritis (ICA) Arthritis Res 2000-08-31 [PMID: 11056679]

Bioinformatics

• Gene Symbol: IL1RN
• Uniprot:
  • P25085()