IL-18 BPa/IL18BP Overexpression Lysate

Images

 
Western Blot: IL18BP Overexpression Lysate (Adult Normal) [NBL1-11920] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IL18BP.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

IL-18 BPa/IL18BP Overexpression Lysate Summary

Description
Transient overexpression lysate of interleukin 18 binding protein (IL18BP), transcript variant E The lysate was created in HEK293T cells, using Plasmid ID RC213871 and based on accession number NM_001039659. The protein contains a C-MYC/DDK Tag.
Gene
IL18BP

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
17.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for IL-18 BPa/IL18BP Overexpression Lysate

  • IL18 BPa
  • IL-18 BPa
  • IL18BP
  • IL-18BP
  • IL18BPa
  • interleukin 18 binding protein
  • interleukin-18-binding protein
  • MC51L-53L-54L homolog gene product
  • tadekinig-alfa

Background

The protein encoded by this gene is an inhibitor of the proinflammatory cytokine IL18. This protein binds to IL18, prevents the binding of IL18 to its receptor, and thus inhibits IL18-induced IFN-gamma production. This protein is constitutively expressed and secreted in mononuclear cells. The expression of this protein can be enhanced by IFN-gamma. An elevated level of this protein is detected in the intestinal tissues of patients with Crohn's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq]. Transcript Variant: This variant (E) differs in the 5' UTR compared to variant A. Variants A, E, F and G encode the same isoform (a, also known as IL-18BPa). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

WB Video Protocol

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Other Available Formats

NBL1-11920

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Bioinformatics

Gene Symbol IL18BP