IL-18 BPa/IL18BP Antibody (136031) [Unconjugated]

• Reactivity: Hu
• Applications: ELISA(Cap)
• Clone: 136031
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-18 BPa/IL18BP Antibody (136031) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL-18 BPa
  Thr31-Gly194 (predicted)
  Accession # O95998
• Specificity: Detects human IL-18 BPa in ELISAs. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) IL-18, recombinant mouse (rm) IL-18 BPc and rmIL-18 BPd is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL18BP
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Capture: Human IL-18 BPa Antibody (Catalog # MAB1192)
  ELISA Detection: Human IL-18 BPa Biotinylated Antibody (Catalog # BAF119)
  Standard: Recombinant Human IL-18 BPa Fc Chimera (Catalog # 119-BP)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-18 BPa/IL18BP Antibody (136031) [Unconjugated]

• IL18 BPa
• IL-18 BPa
• IL18BP
• IL-18BP
• IL18BPa
• interleukin 18 binding protein
• interleukin-18-binding protein
• MC51L-53L-54L homolog gene product
• tadekinig-alfa

Background

Interleukin 18 binding protein (IL-18 BP) is a secreted glycoprotein, which functions as an IL-18 antagonist by binding to IL-18 and blocking its biological activity.
IL‑18 BP bears no amino acid sequence homology to the membrane-associated IL-18 and IL-1 receptor proteins. The gene for human IL-18 BP has been localized to chromosome 11q13. It encodes for at least four isoforms by alternative splicing. The IL-18 BP isoforms a and c each contain one immunoglobulin (Ig)-like C2-type domain while isoforms b and d lack a complete Ig domain. The complete Ig domain has been shown to be essential to the binding and neutralizing properties of the binding proteins. Two isoforms of mouse IL18 BP (c and d) containing the complete Ig domain have also been isolated and shown to neutralize IL-18 bioactivity. Human and mouse IL-18 BPs share approximately 61% amino acid sequence identity. Several poxviruses also encode proteins with sequence similarity to the human and mouse IL-18 BP. Viral IL-18 BPs have been shown to bind and inhibit IL-18 responses and may be involved in modulating host immune responses. The expression of IL-18 BP is markedly upregulated by IFN-gamma , suggesting that IL-18 activity is modulated by a negative feedback mechanism mediated by IL-18 BP.

1. Mühl, H. et al. (2000) Biochem. Biophys. Res. Commun. 267:960.
2. Kim, S-H. et al. (2000) Proc. Nat. Acad. Sci. USA 97:1190.
3. Calderara, S. et al. (2001) Virology 279:22.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-18 BPa/IL18BP Antibody (MAB1192)(2)

Publications using MAB1192

• Linda Bieniussa, Baran Kahraman, Johannes Skornicka, Annemarie Schulte, Johannes Voelker, Sibylle Jablonka et al. Pegylated Insulin-Like Growth Factor 1 attenuates Hair Cell Loss and promotes Presynaptic Maintenance of Medial Olivocochlear Cholinergic Fibers in the Cochlea of the Progressive Motor Neuropathy Mouse Frontiers in Neurology 2022-06-03 [PMID: 35720065]
• Fiona Moghaddas, Ping Zeng, Yuxia Zhang, Heike Schützle, Sebastian Brenner, Sigrun R. Hofmann et al. Autoinflammatory mutation in NLRC4 reveals a leucine-rich repeat (LRR)–LRR oligomerization interface Journal of Allergy and Clinical Immunology 2018-12-01 [PMID: 29778503]

Bioinformatics

• Gene Symbol: IL18BP
• Uniprot:
  • O95998()