IL-17RD/SEF Antibody [Unconjugated]

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HEK293 human cell line transfected with mouse IL-17 RD/SEF was stained with Goat Anti-MouseIL‑17 RD/SEF Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2276, filled histogram) or isotype control antibody ...read more
bEnd.3 mouse endothelioma cell line was stained with Goat Anti-MouseIL‑17 RD/SEF Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2276, filled histogram) or isotype control antibody (Catalog # ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

IL-17RD/SEF Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL‑17 RD/SEF
Gly28-Arg299
Accession # Q8JZL1
Specificity
Detects mouse IL-17 RD/SEF in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human IL-17 RD is observed and less than 1% cross-reactivity with recombinant mouse (rm) IL-17 RC and rmIL-17B R is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL17RD
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF2276 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17RD/SEF Antibody [Unconjugated]

  • FLJ35755
  • IL-17 RD
  • IL17RD
  • IL-17RD
  • IL-17RDIL-17 receptor D
  • IL17Rhom
  • IL17RLM
  • IL17RLMDKFZp434N1928
  • interleukin 17 receptor D
  • interleukin-17 receptor D
  • Interleukin-17 receptor-like protein
  • MGC133309
  • Sef homolog
  • SEF
  • SEFhSef

Background

Interleukin -17 receptor D (IL-17 RD), also known as SEF (similar expression to FGFs), is a type I transmembrane protein that is found in both the cytoplasm and plasma membrane (1‑5). The gene for this protein belongs to a synexpression group originally identified in zebrafish and SEF is expressed along with FGF-3, -8, sprouty-2 (SPRY2) and SPRY4 (6, 7). Due to the presence of an alternate start site, there is one transcript that potentially gives rise to two isoforms. The first is a full-length long form and the second an N-terminally truncated form (2, 5). The significance and expression pattern of the short form are uncertain. The membrane‑bound long form of mouse IL-17 RD is synthesized as a 738 amino acid (aa) precursor protein with a putative 27 aa signal peptide, a 272 aa extracellular domain, a 20 aa transmembrane segment and a 419 aa cytoplastic domain (5). The extracellular domain contains one Ig-like domain and a fibronectin type III motif. The cytoplasmic domain shares homology with the intracellular domains of IL-17 receptor family members and shows one TIR (Toll/IL-1 Receptor) domain and a putative TRAF6-binding motif (2). Natural IL-17 RD has been shown to form homomultimeric complexes (3). The full-length IL-17 RD isoform is expressed in most adult tissues and during embryonic development (3, 5). Functionally, IL-17 RD has been shown to be an inhibitor of FGF signaling. The molecule’s extracellular domain does not seem to be involved. There is an interaction between the intracellular domains of FGFR1/2 and IL-17 RD that blocks ERK dissociation from MEK, thereby interfering with downstream ERK activation of nuclear Elk-1 (8). IL-17 RD has also been reported to interact with TAK1 and induce JNK activation and apoptosis (9). Ligands that interact with the extracellular domain of IL-17 RD have not been identified.
  1. Furthauer, M. et al. (2002) Nat. Cell Biol. 4:170.
  2. Xiong, S. et al. (2003) J. Biol. Chem. 278:50273.
  3. Yang, R-B. et al. (2003) J. Biol. Chem. 278:33232.
  4. Preger, E. et al. (2003) Proc. Natl. Acad. Sci. USA 101:1229.
  5. Lin, W. et al. (2002) Mech. Dev. 113:163.
  6. Tsang, M. et al. (2002) Nat. Cell Biol. 4:165.
  7. Kovalenko, D. et al. (2003) J. Biol. Chem. 278:14087.
  8. Torii, S. et al. (2004) Dev. Cell 7:33.
  9. Yang, X. et al. (2004) J. Biol. Chem. 279:38099.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-17RD/SEF Antibody (AF2276)(4)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: Flow Cytometry, IHC-Fr, Immunoprecipitation, Western Blot.


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Flow Cytometry
(1)
IHC-Fr
(1)
Immunoprecipitation
(1)
Western Blot
(2)
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Mouse
(4)
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Showing Publications 1 - 4 of 4.
Publications using AF2276 Applications Species
D Knizkova, M Pribikova, H Draberova, T Semberova, T Trivic, A Synackova, A Ujevic, J Stefanovic, A Drobek, M Huranova, V Niederlova, O Tsyklauri, A Neuwirth, J Tureckova, O Stepanek, P Draber CMTM4 is a subunit of the IL-17 receptor and mediates autoimmune pathology Nature Immunology, 2022-10-21;0(0):. 2022-10-21 [PMID: 36271145] (Flow Cytometry, Mouse) Flow Cytometry Mouse
ES Park, S Kim, DC Yao, JPJ Savarraj, HA Choi, PR Chen, E Kim Soluble Endoglin Stimulates Inflammatory and Angiogenic Responses in Microglia That Are Associated with Endothelial Dysfunction International Journal of Molecular Sciences, 2022-01-22;23(3):. 2022-01-22 [PMID: 35163148] (Western Blot, Mouse) Western Blot Mouse
Miraoui H, Dwyer A, Sykiotis G, Plummer L, Chung W, Feng B, Beenken A, Clarke J, Pers T, Dworzynski P, Keefe K, Niedziela M, Raivio T, Crowley W, Seminara S, Quinton R, Hughes V, Kumanov P, Young J, Yialamas M, Hall J, Van Vliet G, Chanoine J, Rubenstein J, Mohammadi M, Tsai P, Sidis Y, Lage K, Pitteloud N Mutations in FGF17, IL17RD, DUSP6, SPRY4, and FLRT3 are identified in individuals with congenital hypogonadotropic hypogonadism. Am J Hum Genet, 2013-05-02;92(5):725-43. 2013-05-02 [PMID: 23643382] (IHC-Fr, Mouse) IHC-Fr Mouse
Rong Z, Wang A, Li Z, Ren Y, Cheng L, Li Y, Wang Y, Ren F, Zhang X, Hu J, Chang Z IL-17RD (Sef or IL-17RLM) interacts with IL-17 receptor and mediates IL-17 signaling. Cell Res., 2009-02-01;19(2):208-15. 2009-02-01 [PMID: 19079364] (Immunoprecipitation, Western Blot, Mouse) Immunoprecipitation, Western Blot Mouse

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Bioinformatics

Gene Symbol IL17RD
Uniprot