Recombinant Human IL-17B Protein

SDS-Page: Human IL-17B Protein [NBP2-35044]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human IL-17B Protein Summary

• Description: A non-disulfide-linked homodimer of two 161 amino acid polypeptide chains corresponding to IL-17B Source: E. coli

  Uniprot ID: Q9UHF5

  Amino Acid Sequence: MQPRSPKSKR KGQGRPGPLA PGPHQVPLDL VSRMKPYARM EEYERNIEEM VAQLRNSSEL AQRKCEVNLQ LWMSNKRSLS PWGYSINHDP SRIPVDLPEA RCLCLGCVNP FTMQEDRSMV SVPVFSQVPV RRRLCPPPPR TGPCRQRAVM ETIAVGCTCI F

• Details of Functionality: IL-17B protein is fully biologically active when compared to standard. The ED50 as determined by inducing IL-8 secretion of human HepG2 cells is less than 1.0 ug/ml, corresponding to a specific activity of > 1000 IU/mg
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IL17B
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of IL-17B as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 36.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, with 0.1 % Tween-20 and 3 % Trehalose.
• Preservative: Trehalose
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human IL-17B Protein

• Cytokine Zcyto7
• cytokine-like protein ZCYTO7
• IL17B
• IL-17B
• IL-17Binterleukin-17 beta
• IL20
• IL-20interleukin-17B
• interleukin 17B
• interleukin 20
• Interleukin-20
• MGC138900
• MGC138901
• neuronal interleukin-17 related factor
• Neuronal interleukin-17-related factor
• NIRF
• ZCYTO7interleukin-20

Background

IL-17B, also named IL20, NIRF and ZCYTO7, is belonging to the IL-17 family and it stimulates the release of tumor necrosis factor alpha and IL-1-beta from the monocytic cell line THP-1. IL-17B mRNA is expressed in adult pancreas, small intestine, and stomach. The IL-17 family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17B binds the IL-17B receptor, but not the IL-17 receptor; it is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. This cytokine was reported to stimulate the release of TNF alpha (TNF) and IL1 beta (IL1B) from a monocytic cell line. Immunohistochemical analysis of several nerve tissues indicated that this cytokine is primarily localized to neuronal cell bodies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IL17B
• Uniprot:
  • Q9UHF5()
  • Q9UHF5()
  • Q9UHF5()