Mouse IL-17B ELISA Kit (Colorimetric) Summary
| Description |
Quality control test: Standard Curve. Il17b (Mouse) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Il17b. |
| Sensitivity |
12 pg/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 12% |
| Intra-Assay |
CV% < 10% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
IL17B |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Useful for quantification. |
Packaging, Storage & Formulations
| Storage |
Store at -20 to -80C. Avoid freeze-thaw cycles. |
Kit Components
|
Components
|
- Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For detection antibody and HRP- Streptavidin diluent.
- Assay Diluent D (Item K): 15 ml of 5x concentrated buffer. For Standard/Sample (serum/plasmasamples/cell culture medium) diluent.
- Detection Antibody IL-17B (Item F): 2 vial of biotinylated anti- mouse IL-17B (each vial is enough to assay half microplate).
- HRP-Streptavidin Concentrate (Item G): 8 µl 10,000x concentrated HRP-conjugated streptavidin.
- IL-17B Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-mouse IL-17B.
- Standards (Item C): 2 vials, recombinant mouse IL-17B.
- Stop Solution (Item I): 8 ml of 2 M sulfuric acid.
- TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’-tetramethylbenzidine (TMB) in buffered solution.
- Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Mouse IL-17B ELISA Kit (Colorimetric)
Background
Recently, a number of cytokines belonging to the interleukin (IL)-17 family have been identified. These are IL-17B, IL-17C, and IL-17E. Like IL-17, IL-17B, C, and E are able to induce proinflammatory responses but don't bind to the IL-17 receptor suggesting additional IL-17R related receptors might exist. IL-17E induces activation of NF-kB pathway and like IL-17 also induces production of IL-8. IL-17Rh1 mRNA is highly expressed in kidney and liver and at moderate level in small intestine, colon, and brain.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for IL-17B ELISA Kit (KA1794). (Showing 1 - 1 of 1 FAQ).
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wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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