IL-13R alpha 2 Antibody [Biotin]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

IL-13R alpha 2 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IL‑13 R alpha 2
  Leu22-Lys334
  Accession # O88786
• Specificity: Detects mouse IL-13 R alpha 2 in Western blots. In this format, approximately 1% cross-reactivity with recombinant human IL‑13 R alpha 2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL13RA2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-13R alpha 2 Antibody [Biotin]

• cancer/testis antigen 19
• CD213a2 antigen
• CD213a2
• CT19
• IL-13 R alpha 2
• IL-13 receptor subunit alpha-2
• IL13BP
• IL13R alpha 2
• IL-13R subunit alpha-2
• IL13R
• IL-13R
• IL13RA2
• IL-13Ra2
• IL-13R-alpha-2
• interleukin 13 binding protein
• interleukin 13 receptor alpha 2 chain
• interleukin 13 receptor, alpha 2
• interleukin-13 receptor subunit alpha-2
• Interleukin-13-binding protein

Background

Two type1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha , IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.

The mouse IL-13 R alpha 2 cDNA encodes a 383 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide, a 313 residue extracellular domain, a 22 aa residue transmembrane region and a 27 aa residue cytoplasmic tail. Human and mouse IL-13 R alpha 2 share 59% aa sequence identity. The extracellular domain of IL-13 R alpha 2 is also closely related to that of IL-13 R alpha 1. However, the cytoplasmic domain of IL-13 R alpha 2 lacks the box 1 and box 2 signaling motif and is much shorter than that of IL-13 R alpha 1, suggesting that the two receptors are functionally distinct. IL-13 R alpha 1 has been shown to combine with IL-4 R alpha to form a high-affinity receptor complex capable of transducing both an IL-4-dependent and an IL-13-dependent proliferative signal. The role of IL-13 R alpha 2 in IL-13 signaling remains to be elucidated. The amino-terminal 27 amino acid residues of mouse IL-13 R alpha 2 are identical to that of a soluble mouse IL-13 binding protein purified from mouse serum and urine.

1. Donaldson, D.D. et al. (1998) J. Immunol. 161:2317.
2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-13R alpha 2 Antibody (BAF539)(5)

Publications using BAF539

• Z Li, S Roy, C Ranasinghe IL-13R&alpha2 Regulates the IL-13/IFN-&gamma Balance during Innate Lymphoid Cell and Dendritic Cell Responses to Pox Viral Vector-Based Vaccination Vaccines, 2021-05-01;9(5):. 2021-05-01 [PMID: 34062727] (Flow Cytometry, Mouse)
• S Roy, HY Liu, MI Jaeson, LP Deimel, C Ranasinghe Unique IL-13Ralpha2/STAT3 mediated IL-13 regulation detected in lung conventional dendritic cells, 24?h post viral vector vaccination Sci Rep, 2020-01-23;10(1):1017. 2020-01-23 [PMID: 31974500] (Flow Cytometry, ICC, Mouse)
• Makiko Okubo M2-polarized macrophages contribute to neovasculogenesis, leading to relapse of oral cancer following radiation Sci Rep, 2016-06-08;6(0):27548. 2016-06-08 [PMID: 27271009] (Flow Cytometry, Western Blot, Mouse)
• Lee , Gerald B, Brandt , Eric B, Xiao , Chang, Gibson , Aaron M, Le Cras , Timothy, Brown , Lou Ann, Fitzpatrick , Anne M, Khurana Hershey , Gurjit K Diesel exhaust particles induce cysteine oxidation and s-glutathionylation in house dust mite induced murine asthma. PLoS ONE, 2013-03-29;8(3):e60632. 2013-03-29 [PMID: 23555996] (ELISA Development (Detection), Mouse)
• Chen W, Tabata Y, Gibson AM, Daines MO, Warrier MR, Wills-Karp M, Hershey GK Matrix metalloproteinase 8 contributes to solubilization of IL-13 receptor alpha2 in vivo. J. Allergy Clin. Immunol., 2008-08-09;122(3):625-32. 2008-08-09 [PMID: 18694590] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: IL13RA2
• Uniprot:
  • O88786()