IL-13R alpha 2: Novus Biologicals

AF146

IL-13R alpha 2 Antibody [Unconjugated]

100 μg

AF146

HAF017). A specific band was detected for IL-13 Ra2 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human placenta tissue and human testis tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human IL-13 Ra2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF146) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody <a class=

HAF017). A specific band was detected for IL-13 Ra2 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human placenta tissue and human testis tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human IL-13 Ra2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF146) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody HAF017). A specific band was detected for IL-13 Ra2 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

IL-13 Ra2 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human IL-13 Ra2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF146) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown <a class=

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of human placenta tissue and human testis tissue, loaded at 0.2 mg/mL. A specific band was detected for IL-13 Ra2 at approximately 59 kDa (as indicated) using 10 µg/mL of Goat Anti-Human IL-13 Ra2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF146) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

Staining Membrane-associated Proteins." class="big_lightbox">

A375 human melanoma cell line was stained with Goat Anti-Human IL-13 Ra2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF146, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram). View our protocol for <a class=NoLineLink href=

Staining Membrane-associated Proteins." class="big_thumb" />

Evaluation of additional commercially available B-D13 antibodies.(A) PBT008 cells that had been cultured overnight in media alone (black histogram) versus cytokine (TNF; red histogram) conditions, (B) Parental 293T cells or 293T cells engineered to express either VCAM-1 or IL13R alpha 2, and (C) U251T cells were stained with VCAM-1-PE, AF146 or various B-D13 reagents – two lots of PE-conjugated B-D13 antibody (B-D13-PE; Cell Sciences) and two to three unconjugated B-D13 antibodies (B-D13-unc) purchased from either Cell Sciences (Cell Sci), Abcam or Santa Cruz as indicated. (B, C) Black histograms represent staining with istoype control antibody or SA-PE alone. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0095123), licensed under a CC-BY license. Not internally tested by R&D Systems.

Deletion of the cytoplasmic domain of IL-13R alpha 2 resulted in a loss of physical interaction with EGFRvIII and enhanced proliferation is abolished. a Whole-cell lysates prepared from stable cell line Gli36.IL-13R alpha 2/EGFRvIII cells were used for immunoprecipitation with anti-IL-13R alpha 2 antibody, then immunoprobed with an anti-EGFR antibody. IgG served as control while unprecipitated extracts serve as input. b Similar cell lysates were reverse immunoprecipitated with anti-EGFR antibody, then immunoprobed with an anti-IL13R alpha 2antibody. Lysates from Gli36.EGFRvIII served as additional control c Gli36.IL-13R alpha 2/EGFRvIII cell lysates were immunoprecipitated with anti-EGFR antibody, then immunoprobed with anti-Grb antibody. To further examine the domains of interaction, IL-13R alpha 2 and EGFR mutants were used. Gli36.EGFRvIII cells were first transfected with pIRESneo2 (Vector), IL-13R alpha 2 full length (Wild-type) and IL-13R alpha 2 Cyt tail deleted constructs (Mutant) and then analyzed by d cell proliferation assay at the indicated time points, f co-immunoprecipitation, and h PLA assays. Findings were validated using Gli36.IL-13R alpha 2 cells transiently transfected with vector (CTRL), full length/wild-type EGFRvIII, DK, and DY3 mutants. e proliferation outputs, g co-immunoprecipitation, i and PLA assay were performed. j represent the corresponding positive and negative controls. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29203859), licensed under a CC-BY license. Not internally tested by R&D Systems.

GBM patients co-expressing EGFR and IL-13R alpha 2 correlate to poor survival where the overexpression of IL-13R alpha 2 alone leads to enhance cell migration but not proliferation. Kaplan−Meier survival analysis of a all gliomas patients; b GBM patients from REMBRANDT database from National Cancer Institute (USA). Patients overexpressing EGFR mRNA by 2-fold (blue) with high (red), intermediate (yellow) and low (green) levels of IL-13R alpha 2 expression were shown. The log-rank p-values were indicated. c Kaplan−Meier survival plots for patients expressing high YKL-40 mRNA levels TCGA. High IL-13R alpha 2 expression group (red) and low IL-13R alpha 2 expression group (blue) were determined by aggregating all patients whose z-score normalized expression was above or below 0, respectively (Log-rank test p-value = 0.0374). Immunoblotting analysis showed the expression of EGFR and IL-13R alpha 2 protein levels were determined from d a panel of 10 patient-derived GBM e and the isogenic cell lines generated from Gli36 glioma cells. Pan-actin or beta tubulin served as internal loading controls. f Cell proliferation and g Cell cycle analysis were performed with Gli36 and Gli36.IL-13R alpha 2 cells h Soft agar colony formation assay was performed, Gli36.EGFRvIII was used as a positive control. i In vitro migration and j invasion assays were determined in Gli36 and Gli36.IL-13R alpha 2 cells. All data are represented as mean ± SEM, unpaired t-test **p < 0.01; ***p < 0.001; NS not significant. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29203859), licensed under a CC-BY license. Not internally tested by R&D Systems.

The cytokine-induced cell surface antigen recognized by B-D13-PE does not bind IL-13.(A) D283 cells engineered to express IL13R alpha 2 (D283- IL13R alpha 2) (blue histograms) and D283 parental (black histograms) cells were evaluated by flow cytometry for expression of constitutive IL13R alpha 2 using the IL13R alpha 2-specific antibody AF146, and biotinylated recombinant human IL-13 (IL13-bio) followed by PE-conjugated strepavidin (SA-PE). Data are representative of 2 separate experiments. (B) U251T (grown in the absence of cytokines) were evaluated by flow cytometry for constitutive IL13R alpha 2 expression using AF146, and for binding to IL13-bio/SA-PE in the presence and absence of 10-fold molar excess of recombinant human IL-4 or IL-13. Black histograms represent staining with istoype control antibody or SA-PE alone. Data are representative of 2 separate experiments. (C) THP-1 and PBT008 grown in media alone (black histograms) or induced overnight with TNF and IL-4 (red histograms) were analyzed by flow cytometry for expression of constitutive IL13R alpha 2 (AF146), for expression of the induced antigen (B-D13-PE), and for binding to IL13-bio/SA-PE. Data are representative of 3 separate experiments. (D) IL13-zetakine+ CD8+ CTL recognize and kill U251T glioma targets expressing constitutive IL13R alpha 2 (AF146-positive), but not cytokine-induced PBT003 cells (B-D13-positive). Percentage specific lysis (mean ± S.D.) of triplicate wells is depicted. *, p≤0.0002 using an unpaired Student's t-test to compare U251T vs. PBT003-4 targets. #, p>0.05 using an unpaired Student's t-test to compare PBT003-4 targets with and without overnight cytokine stimulation. Data are representative of at least 2 separate experiments. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0095123), licensed under a CC-BY license. Not internally tested by R&D Systems.

Differential recognition of constitutively-expressed versus cytokine-induced IL13R alpha 2 by commercially available anti-IL13R alpha 2 antibodies.Flow cytometric analysis of monocytic line THP-1 and various glioma lines with (A) B-D13-PE (Cell Sciences) or (B) AF146 (R&D Systems) reagents for media alone (black histogram) and cytokine (TNF/IL-4 or TNF/IL-13 overnight; red histogram) conditions. Isotype (iso-PE) and mouse anti-goat-FITC controls are shown as grey histograms. Percent positive and relative fluorescent index (RFI) of MFI cytokine/MFI media is reported for each histogram. (C) Flow cytometric detection of IL13R alpha 2 for D283 cells engineered to express IL13R alpha 2 (D283-IL13R alpha 2; blue histogram) and D283 parental (black histogram) stained with AF146 or B-D13-PE antibodies. All data are representative of more than three experiments each. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0095123), licensed under a CC-BY license. Not internally tested by R&D Systems.

IL-13R alpha 2 mediate greater tumorigenic potential with EGFRvIII, and not WT EGFR. a Tumor volume b and tumor weight of tetracycline regulatable U251 gliomas (U251-E6 and U251-E18 was examined in vivo. Bars depict the mean values and error bars represent 95% confidence intervals. P-values were calculated using ANOVA with Tukey’s multiple comparison test *p < 0.05; **p < 0.01; ***p < 0.001. Photomicrographs of represented collected tumors are shown. c Immunoblot analysis of proteins from U251-E6 and -E18 tumor lysates in the presence or absence of tetracycline with the indicated antibodies. One representative tumor under each of the uninduced and induced conditions was shown. U251MG whole-cell lysate served as positive control for IL-13R alpha 2. d Kaplan−Meier survival curves of mice bearing U251-E6 and U251-18 tumors **p < 0.0039. Kaplan−Meier survival plots for patients expressing e high EGFR mRNA levels (excluding EGFRvIII) or f high EGFRvIII mRNA levels from TCGA database. High IL-13R alpha 2 expression (red) and low IL-13R alpha 2 expression (blue) were determined by aggregating all patients whose z-score normalized expression was above or below 0, respectively. g Schematic model showing signal transduction pathway co-induced by IL-13R alpha 2 and EGFRvIII. Overexpression of IL-13R alpha 2 in human gliomas increases cell migration and invasion through the activation of MMP-2, vimentin. Amplification of EGFRvIII promotes the co-interaction of both receptors mediating an increase in tyrosine kinase activities and a preferential activation of RAS-MEK-ERK and STAT3 pathways leading to aberrant cellular proliferation. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29203859), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Cytokine induction of B-D13 antigen is dependent on mRNA transcription and translation.(A) IL13R alpha 2 mRNA levels quantified by qPCR for U251T, PBT003-4, PBT008, and THP-1 cells after overnight culture in media, TNF/IL-4, or TNF/IL-13. mRNA levels were normalized to housekeeping genes (ACTB, UBC, GAPDH and RPLP0). (B, C) B-D13-PE immunoreactivity of PBT008 and THP-1 cells treated with increasing concentrations of (B) transcription blocker Actinomycin D (ActD) (0, 0.06, 0.1, 0.3 µg/ml) or (C) translation blocker Cycloheximide (CHX) (0, 2.5, 5, 10 µg/ml), then either cultured in media alone (black histograms) or stimulated with TNF/IL-4 (red histograms) for 5 hours. All data are representative of 2 separate experiments. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0095123), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Genetic Strategies Validation. </b>Enhanced cellular proliferation mediated by IL-13R alpha 2 is specific to EGFRvIII, and not WT EGFR. a U251-E6 or c U251-E18 cells were treated with or without tetracycline (Tet). At indicated time points, immunoblot analysis was carried out. Gli36, Gli36.EGFRvIII cell lysates were included as negative or positive controls for EGFRvIII, respectively. Growth kinetics of b U251-E6 and d U251-E18 was determined by CCK-8 assay. Percent cell viability was normalized to day 1 (without induction). All data are represented as mean ± SEM. Unpaired t-test ***p < 0.001, NS. not significant. e Co-immunoprecipitation was performed in stable cell lines Gli36.IL-13R alpha 2/wtEGFR as well as U251MG-E6 (i.e. wtEGFR) cells at 48 h post tetracycline induction with the indicated antibodies. Gli36.IL-13R alpha 2/EGFRvIII served as positive controls. f The interaction between endogenous wtEGFR and IL-13R alpha 2 was shown in primary wtEGFR-positive GBM patient tumor derived from Mayo clinic, and IgG served as positive and negative controls respectively. Knockdown of IL-13R alpha 2 in cell line or patient-derived GBM samples expressing g wtEGFR or h EGFRvIII. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29203859), licensed under a CC-BY license. Not internally tested by R&D Systems.

B-D13 reagent appears to contain two distinct monoclonal antibodies.Soluble receptor competition study evaluating the specificity of IL13R alpha 2 and VCAM-1 recognition by the B-D13-unc antibodies (Cell Sciences and Santa Cruz) using (A) PBT008 cells cultured overnight in media alone (black histogram) versus cytokine (TNF; blue histogram); or (B) IL13R alpha 2-expressing U251T cells (blue histogram). Cells were stained with the indicated antibody that was pre-incubated with soluble recombinant human IL13R alpha -Fc (purple histograms) or VCAM-1-Fc (green histograms). Relative fluorescence index (RFI) compared to staining without the soluble competitors (i.e., the control/blue histograms) are indicated in each histogram. (C) Unconjugated B-D13 antibodies from Cell Sciences (top) and Abcam (bottom) were reduced and analyzed by LC/MS. Shown is the spectra of the deconvoluted protein masses depicting two distinct mass species for both the heavy and light chains. (D) Extracted ion chromatograms (EIC) for the two light chain species, of the Cell Sciences B-D13-unc reagent from (C). Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0095123), licensed under a CC-BY license. Not internally tested by R&D Systems.

IL13R alpha 2 expression is associated with mesenchymal gene expression in low-passage primary glioma cell lines.Flow cytometry analyses of (A) IL13R alpha 2-negative glioma cell lines (PBT003-4, PBT008, PBT009, PBT022), and (B) IL13R alpha 2-positive glioma cell lines (PBT015, PBT017-4, PBT030, PBT036, and U251T) for expression of the proneural marker CD133 and mesenchymal markers CD44 and CD54/ICAM-1 (grey histograms). Solid lines show isotype and secondary control antibodies. (C) Comparison of mean fluorescence intensity (MFI) of IL13R alpha 2-negative and IL13R alpha 2-positive glioma cell lines shown in panels A and B. (D-E) Distribution plots of Affymetrix gene array expression analyses of (D) IL13R alpha 2-negative and (E) IL13R alpha 2-positive glioma cell lines for mesenchymal, classical, neural and proneural signature gene expression. (F) Correlation of mesenchymal (MES) versus proneural (PN) average gene expression with RMA normalized IL13R alpha 2 expression in glioma cell lines. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24204956), licensed under a CC-BY license. Not internally tested by R&D Systems.

MAB6141) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑13 R alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF146) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Human APRIL/TNFSF13 was serially diluted and captured by Mouse Anti-Human IL‑13 R alpha 2 Monoclonal Antibody (Catalog # <a class=

MAB6141) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑13 R alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF146) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF146U-100UG
Alexa Fluor 405	AF146V-100UG
Alexa Fluor 488	AF146G-100UG
Alexa Fluor 532	AF146X-100UG
Alexa Fluor 594	AF146T-100UG
Alexa Fluor 647	AF146R-100UG
Alexa Fluor 700	AF146N-100UG
Alexa Fluor 750	AF146S-100UG
Alexa Fluor Plus 405	AF146AFP405-100UG
Alexa Fluor Plus 488	AF146AFP488-100UG
Alexa Fluor Plus 555	AF146AFP555-100UG
Alexa Fluor Plus 594	AF146AFP594-100UG
Alexa Fluor Plus 647	AF146AFP647-100UG
Alexa Fluor Plus 680	AF146AFP680-100UG
Allophycocyanin	FAB614A
Biotin	BAF614
CoraFluor 1	AF146CL1
DyLight 350	AF146D
DyLight 405	AF146E
DyLight 488	AF146K
DyLight 550	AF146L
DyLight 594	AF146M
DyLight 650	AF146W
DyLight 680	AF146Y
DyLight 755	AF146Z
Fluorescein	FAB614F
HRP	AF146H
Janelia Fluor 525	AF146JF525
Janelia Fluor 549	AF146I
Janelia Fluor 646	AF146J
Janelia Fluor 669	AF146JF669
PE	AF146P
PerCP	AF146C
mFluor Violet 450 SE	AF146MFV450
mFluor Violet 500 SE	AF146MFV500
mFluor Violet 610 SE	AF146MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB614F
Carrier Free	FAB614A
Carrier Free	AF146-SP

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AF539

IL-13R alpha 2 Antibody [Unconjugated]

(13 Publications)

100 μg

AF539

NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL-13 Ra2 was detected in immersion fixed mouse splenocytes using Mouse IL-13 Ra2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF539) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

IL-13 Ra2 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse IL-13 Ra2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF539) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF539U-100UG
Alexa Fluor 405	AF539V-100UG
Alexa Fluor 488	AF539G-100UG
Alexa Fluor 532	AF539X-100UG
Alexa Fluor 594	AF539T-100UG
Alexa Fluor 647	AF539R-100UG
Alexa Fluor 700	AF539N-100UG
Alexa Fluor 750	AF539S-100UG
Alexa Fluor Plus 405	AF539AFP405-100UG
Alexa Fluor Plus 488	AF539AFP488-100UG
Alexa Fluor Plus 555	AF539AFP555-100UG
Alexa Fluor Plus 594	AF539AFP594-100UG
Alexa Fluor Plus 647	AF539AFP647-100UG
Alexa Fluor Plus 680	AF539AFP680-100UG
Allophycocyanin	AF539A
Biotin	BAF539
CoraFluor 1	AF539CL1
DyLight 350	AF539D
DyLight 405	AF539E
DyLight 488	AF539K
DyLight 550	AF539L
DyLight 594	AF539M
DyLight 650	AF539W
DyLight 680	AF539Y
DyLight 755	AF539Z
FITC	AF539F
HRP	AF539H
Janelia Fluor 525	AF539JF525
Janelia Fluor 549	AF539I
Janelia Fluor 646	AF539J
Janelia Fluor 669	AF539JF669
PE	AF539P
PerCP	AF539C
mFluor Violet 450 SE	AF539MFV450
mFluor Violet 500 SE	AF539MFV500
mFluor Violet 610 SE	AF539MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF539-SP

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MAB614

IL-13R alpha 2 Antibody (83834) [Unconjugated]

(8 Publications)

100 μg

MAB614

HAF018). A specific band was detected for IL-13 Ra2 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human placenta tissue and human testis tissue. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human IL-13 Ra2 Monoclonal Antibody (Catalog # MAB614) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF018). A specific band was detected for IL-13 Ra2 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human placenta tissue and human testis tissue. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human IL-13 Ra2 Monoclonal Antibody (Catalog # MAB614) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-13 Ra2 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Evaluation of apoptosis and microvessel density after sunitinib treatment in xenograft model derived from cell lines.MVD was decreased by sunitinib treatment of each xenograft tumor derived from (A) 786-O or (B) Caki-1 subclones regardless of IL13RA2 expression level. MVD was determined from CD31 staining using Image J software. Statistical analysis was performed using the Students’ t-test (*P < 0.01). Immunoblot analysis of (C) 786-O subclones and (D) Caki-1 subclones. IL13RA2 expression was negatively correlated with the phosphorylation of STAT6. Whole cell extracts were immunoblotted using the indicated antibodies. ssDNA staining of xenograft tumors derived from (E) 786-O subclones and (B) Caki-1 subclones treated with sunitinib or vehicle only. Apoptosis was assessed by calculating the ssDNA positivity rate. Statistical analysis was performed using the Students’ t-test (*P < 0.05, **P < 0.01). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26114873), licensed under a CC-BY license. Not internally tested by R&D Systems.

Overexpression of IL13RA2 leads to acquired resistance to sunitinib and shRNA-mediated IL13RA2 knockdown induces sensitivity to sunitinib.(A) Immunoblot analysis of 786-O subclones infected with retrovirus encoding mock or WT IL13RA2. Whole cell extracts were immunoblotted using the indicated antibodies. Sequential changes in subcutaneous xenograft tumors from 786-O subclones infected with (B) mock or (C) WT IL13RA2 treated with sunitinib and vehicle (control). Each time point represents the mean ± SE of tumor volume in each group. The difference in tumor size between the treatment group and control was statistically significant in 786-O-mock cells but not statistically significant in 786-O-IL13RA2 cells (*P < 0.05, n.s.: not significant; two-way repeated ANOVA). The horizontal arrow bars indicate the periods of sunitinib administration. (D) Immunoblot analysis of Caki-1 subclones infected with lentivirus encoding scrambled or IL13RA2 shRNA. Whole cell extracts were immunoblotted using the indicated antibodies. Sequential changes of subcutaneous xenograft tumors from a Caki-1 subclone infected with (E) scrambled or (F) IL13RA2 shRNA treated with sunitinib and vehicle (control). Each time point represents the mean ± SE of tumor volume in each group. Day 0 is the first day of sunitinib administration 4 weeks after transplantation. The difference in tumor size between the treatment group and control was not significant in Caki-1-sh-scrambled cells but statistically significant in Caki-1-sh-IL13RA2 cells (n.s.: not significant, *P < 0.05; two-way repeated ANOVA). The arrow bars indicate the period of sunitinib administration. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26114873), licensed under a CC-BY license. Not internally tested by R&D Systems.

Evaluation of IL13RA2 mRNA and protein expression.(A) Evaluation of IL13RA2 mRNA expression in KURC1 and KURC2 xenograft tumors treated with sunitinib or vehicle by qPCR. All samples were prepared in triplicate and data are presented as the mean ± SE from indicated number of samples. Columns, mean; bar, SE. The difference in the mRNA expression levels between the sunitinib-treated group and control or sensitive group in KURC1 was statistically significant (*P < 0.01; Students’ t-test). There was no significant difference in KURC2 groups. (B) Immunohistochemical staining of IL13RA2 in KURC1 xenograft tumors. Scale bar, 50 μm. (C) IL13RA2 expression in human ccRCC tumors with the response to sunitinib treatment evaluated by immunohistochemistry. ccRCC tumor samples were collected from patients prior to sunitinib treatment. Left: representative pictures of immunohistochemistry sections of tumors showing none, weak, or strong staining for IL13RA2. Right: ratio of IL13RA2 expression pattern and correlation of the response to sunitinib treatment. Scale bar, 100 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26114873), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB614U-100UG
Alexa Fluor 405	FAB614V-100UG
Alexa Fluor 488	FAB614G-100UG
Alexa Fluor 532	FAB614X-100UG
Alexa Fluor 594	FAB614T-100UG
Alexa Fluor 647	FAB614R-100UG
Alexa Fluor 700	FAB614N-100UG
Alexa Fluor 750	FAB614S-100UG
Alexa Fluor Plus 405	FAB614AFP405-100UG
Alexa Fluor Plus 488	FAB614AFP488-100UG
Alexa Fluor Plus 555	FAB614AFP555-100UG
Alexa Fluor Plus 594	FAB614AFP594-100UG
Alexa Fluor Plus 647	FAB614AFP647-100UG
Alexa Fluor Plus 680	FAB614AFP680-100UG
Biotin	FAB614B
CoraFluor 1	FAB614CL1
DyLight 350	FAB614D
DyLight 405	FAB614E
DyLight 488	FAB614K
DyLight 550	FAB614L
DyLight 594	FAB614M
DyLight 650	FAB614W
DyLight 680	FAB614Y
DyLight 755	FAB614Z
HRP	FAB614H
Janelia Fluor 525	FAB614JF525
Janelia Fluor 549	FAB614I
Janelia Fluor 646	FAB614J
Janelia Fluor 669	FAB614JF669
PE	FAB614P
PerCP	FAB614C
mFluor Violet 450 SE	FAB614MFV450
mFluor Violet 500 SE	FAB614MFV500
mFluor Violet 610 SE	FAB614MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB614-SP
Carrier Free	MAB614-500

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539-IR

Recombinant Mouse IL-13 R alpha 2 Fc Chimera Protein, CF

(11 Publications)

100 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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7147-IR

Recombinant Human IL-13 R alpha 2 Fc Chimera (CHO), CF

(7 Publications)

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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DY614

Human IL-13 R alpha 2 DuoSet ELISA, 15 Plate

(5 Publications)

1 Kit (for 15 Plates)

DY614

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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H00003598-M01

IL-13R alpha 2 Antibody (2E10) - Azide and BSA Free

(1 Publication)

0.1 mg

H00003598-M01

Western Blot: IL-13R alpha 2 Antibody (2E10) [H00003598-M01] - Analysis of IL13RA2 expression in transfected 293T cell line by IL13RA2 monoclonal antibody (M01), clone 2E10.Lane 1: IL13RA2 transfected lysate(44.176 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: IL-13R alpha 2 Antibody (2E10) [H00003598-M01] - Detection limit for recombinant GST tagged IL13RA2 is approximately 0.1ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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MAB6141

IL-13R alpha 2 Antibody (83807)

(2 Publications)

100 μg

MAB6141

DY990). Goat Anti-Human IL‑13 R alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF146) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

DY990). Goat Anti-Human IL‑13 R alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF146) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Human IL-13 R alpha 2 was serially diluted and captured by Mouse Anti-Human IL‑13 R alpha 2 Monoclonal Antibody (Catalog # MAB6141) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑13 R alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF146) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB6141U-100UG
Alexa Fluor 405	FAB6141V-100UG
Alexa Fluor 488	FAB6141G-100UG
Alexa Fluor 532	FAB6141X-100UG
Alexa Fluor 594	FAB6141T-100UG
Alexa Fluor 647	FAB6141R-100UG
Alexa Fluor 700	FAB6141N-100UG
Alexa Fluor 750	FAB6141S-100UG
Alexa Fluor Plus 405	FAB6141AFP405-100UG
Alexa Fluor Plus 488	FAB6141AFP488-100UG
Alexa Fluor Plus 555	FAB6141AFP555-100UG
Alexa Fluor Plus 594	FAB6141AFP594-100UG
Alexa Fluor Plus 647	FAB6141AFP647-100UG
Alexa Fluor Plus 680	FAB6141AFP680-100UG
Allophycocyanin	FAB6141A
Biotin	FAB6141B
CoraFluor 1	FAB6141CL1
DyLight 350	FAB6141D
DyLight 405	FAB6141E
DyLight 488	FAB6141K
DyLight 550	FAB6141L
DyLight 594	FAB6141M
DyLight 650	FAB6141W
DyLight 680	FAB6141Y
DyLight 755	FAB6141Z
FITC	FAB6141F
HRP	FAB6141H
Janelia Fluor 525	FAB6141JF525
Janelia Fluor 549	FAB6141I
Janelia Fluor 646	FAB6141J
Janelia Fluor 669	FAB6141JF669
PE	FAB6141P
PerCP	FAB6141C
mFluor Violet 450 SE	FAB6141MFV450
mFluor Violet 500 SE	FAB6141MFV500
mFluor Violet 610 SE	FAB6141MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB6141-SP
Carrier Free	MAB6141-500

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NBP3-32466

IL-13R alpha 2 Antibody (PSH03-85)

100 ul

NBP3-32466

Flow Cytometry: IL-13R alpha 2 Antibody (PSH03-85) [NBP3-32466] - Flow cytometric analysis of A375 (positive) and U-937 (negative) cells labeling IL-13R alpha 2.<br/><br/> Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32466, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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M13RA2

Mouse IL-13 R alpha 2 Quantikine ELISA Kit

(1 Publication)

1 Kit

M13RA2

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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NBP3-21312

IL-13R alpha 2 Antibody - BSA Free

100 ul

NBP3-21312

Staining of human cell line U-2 OS shows localization to cell junctions.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-21312-25ul

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NBP3-21272

IL-13R alpha 2 Antibody - BSA Free

100 ul

NBP3-21272

Staining of human cell line BJ shows localization to nucleoplasm & plasma membrane.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-21272-25ul

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Has Sample Size

Promotional Item

Species Glossary

IL13RA2 Products By Gene Name

IL-13R alpha 2 Antibody [Unconjugated]

IL-13R alpha 2 Antibody [Unconjugated]

IL-13R alpha 2 Antibody (83834) [Unconjugated]

Recombinant Mouse IL-13 R alpha 2 Fc Chimera Protein, CF

Recombinant Human IL-13 R alpha 2 Fc Chimera (CHO), CF

Human IL-13 R alpha 2 DuoSet ELISA, 15 Plate

IL-13R alpha 2 Antibody (2E10) - Azide and BSA Free

IL-13R alpha 2 Antibody (83807)

IL-13R alpha 2 Antibody (PSH03-85)

Mouse IL-13 R alpha 2 Quantikine ELISA Kit

IL-13R alpha 2 Antibody - BSA Free

IL-13R alpha 2 Antibody - BSA Free