IL-13R alpha 1 Antibody [Biotin]

Human peripheral blood granulocytes were stained with Human IL‑13 R alpha 1 Biotinylated Antigen Affinity‑purified Polyclonal Antibody (Catalog # BAF152, filled histogram) or control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).

• Reactivity: Hu
• Applications: WB, Flow, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

IL-13R alpha 1 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL‑13 R alpha 1
  Ala27-Thr343 (Thr130Ile)
  Accession # Q5JSL4
• Specificity: Detects human IL-13 R alpha 1 in ELISAs and Western blots. In sandwich ELISAs, less than 1% cross-reactivity with recombinant human (rh) IL‑13 R alpha 2, recombinant mouse (rm) IL-13 R alpha , rhIL-4 R, rhIL-5 R alpha , and rhIL-9 R is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL13RA1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human IL-13 R alpha 1 Antibody (Catalog # MAB146)
  ELISA Detection: Human IL-13 R alpha 1 Biotinylated Antibody (Catalog # BAF152)
  Standard: Recombinant Human IL-13 R alpha 1 Fc Chimera (Catalog # 146-IR)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-13R alpha 1 Antibody [Biotin]

• bB128O4.2.1 (interleukin 13 receptor, alpha 1)
• Cancer/testis antigen 19
• CD213a1 antigen
• CD213a1
• CT19
• IL-13 R alpha 1
• IL13 receptor alpha-1 chain
• IL-13 receptor subunit alpha-1
• IL13R alpha 1
• IL-13R subunit alpha-1
• IL13R
• IL13RA
• IL-13Ra
• IL13RA1
• IL-13Ra1
• IL-13R-alpha-1
• interleukin 13 receptor, alpha 1
• interleukin-13 receptor subunit alpha-1
• NR4

Background

Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha , IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.

The human IL-13 R alpha 1 was originally cloned based on sequence homology to the mouse IL-13 R alpha 1. The IL-13 R alpha 1 cDNA encodes a 427 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide, a 324 aa residue extracellular domain, a 23 aa residue transmembrane region, and a 59 aa residue cytoplasmic tail. Human and mouse IL-13 R alpha 1 share 76% aa sequence identity. The extracellular domain of IL-13 R alpha 1 is also closely related to that of IL‑13 R alpha 2. IL‑13 R alpha 1 has been shown to combine with the IL-4 R alpha to form a high-affinity receptor complex capable of transducing an IL-13-dependent proliferative signal. The role of IL-13 R alpha 2 in IL-13 signaling remains to be elucidated.

1. Caput, D. et al. (1996) J. Biol. Chem. 271:16921.
2. Donaldson, D.D. et al. (1998) J. Immunol. 161:2317.
3. Aman, M.J. et al. (1996) J. Biol. Chem. 271:29265.
4. Hilton, D.J. et al. (1996) Proc. Natl. Acad. Sci. USA 93:497.
5. Zhang, J.G. et al. (1997) J. Biol. Chem. 272:9474.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-13R alpha 1 Antibody (BAF152)(2)

Publications using BAF152

• LA Stern, S Gholamin, I Moraga, X Yang, S Saravanaku, JR Cohen, R Starr, B Aguilar, V Salvary, JC Hibbard, A Kalbasi, JK Shepphird, J O'Hearn, KC Garcia, CE Brown Engineered IL13 variants direct specificity of IL13Ralpha2-targeted CAR T cell therapy Proceedings of the National Academy of Sciences of the United States of America, 2022-08-08;119(33):e2112006119. 2022-08-08 [PMID: 35939683] (Flow Cytometry, Yeast)
• Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands. J. Immunol., 2004-12-01;173(11):6592-602. 2004-12-01 [PMID: 15557149] (Flow Cytometry, ICC, Human)

Bioinformatics

• Gene Symbol: IL13RA1
• Uniprot:
  • Q5JSL4()