213-ILB) stimulates prolif-eration in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-13 Ra1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF152). The ND₅₀ is typically 1.00-12.0 µg/mL." class="big_lightbox"> 213-ILB) stimulates prolif-eration in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-13 Ra1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF152). The ND₅₀ is typically 1.00-12.0 µg/mL." alt="Recombinant Human IL-13 (213-ILB) stimulates prolif-eration in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-13 Ra1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF152). The ND₅₀ is typically 1.00-12.0 µg/mL." class="big_thumb" />

AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (F0107)." class="big_lightbox"> AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (F0107)." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in myocardial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in myocardial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

146-IR) was serially diluted and captured by Mouse Anti-Human IL‑13 R alpha 1 Monoclonal Antibody (Catalog # MAB146) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑13 R alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF152) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 146-IR) was serially diluted and captured by Mouse Anti-Human IL‑13 R alpha 1 Monoclonal Antibody (Catalog # MAB146) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑13 R alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF152) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

HAF007). A specific band was detected for IL-13 Ra1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF007). A specific band was detected for IL-13 Ra1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human heart tissue and human skeletal muscle tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IL-13 Ra1 Monoclonal Antibody (Catalog # MAB1462) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-13 Ra1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

FAB1137A) and (B) Mouse Anti-Human IL-13 Ra1 Monoclonal Antibody (Catalog # MAB1462, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." class="big_lightbox"> FAB1137A) and (B) Mouse Anti-Human IL-13 Ra1 Monoclonal Antibody (Catalog # MAB1462, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B)." class="big_thumb" />

146-IR) was serially diluted and captured by Mouse Anti-Human IL‑13 R alpha 1 Monoclonal Antibody (Catalog # MAB146) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑13 R alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF152) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 146-IR) was serially diluted and captured by Mouse Anti-Human IL‑13 R alpha 1 Monoclonal Antibody (Catalog # MAB146) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑13 R alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF152) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human IL‑13 R alpha 1 Fc Chimera (Catalog # 146-IR) was serially diluted and captured by Mouse Anti-Human IL‑13 R alpha 1 Monoclonal Antibody (Catalog # MAB146) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑13 R alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF152) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />