Monkey IL-12 p70/IL-12A ELISA Kit (Colorimetric)

Sandwich ELISA: Monkey IL-12 p70/IL-12A ELISA Kit (Colorimetric) [NBP1-92710] - Representative standard curve for monkey IL-12p70 ELISA. Monkey IL-12p70 was diluted in serial 2-fold steps in Sample Diluent. Do not use this standard curve to derive test results. A standard curve must be run for each group of microwell strips assayed.

Product Discontinued

Monkey IL-12 p70/IL-12A ELISA Kit (Colorimetric) Summary

• Description: The monkey IL-12 p70 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of monkey IL-12 p70.
• Specificity: The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a monkey IL-12p70 positive serum. There was no crossreactivity detected
• Standard Curve Range: 3.9 - 250 U/mL
• Sensitivity: 2.6 U/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 4.8%
• Intra-Assay: CV% < 5.0%
• Spike Recovery: The overall mean recovery was 105%. Please see protocol for more specific Spike Recovery details.
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL12A

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Alternate Names for Monkey IL-12 p70/IL-12A ELISA Kit (Colorimetric)

• CLMF p35
• Cytotoxic lymphocyte maturation factor 35 kDa subunit
• IL12 p70
• IL-12 p70
• IL-12 subunit p35
• IL-12, subunit p35
• interleukin 12, p35
• interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35)
• interleukin-12 alpha chain
• interleukin-12 subunit alpha
• natural killer cell stimulatory factor 1, 35 kD subunit
• NF cell stimulatory factor chain 1
• NK cell stimulatory factor chain 1
• NKSF1
• p35

Background

IL-12 exerts a variety of biological effects on T and natural killer cells. Apart from promotion of Th1 development and its ability to promote cytolytic activity it mediates some of its physiological activities by acting as a potent inducer of IFN-gamma production and the stimulation of other cytokines from peripheral blood T and NK cells. IFN-gamma then enhances the ability of the phagocytic cells to produce IL-12 and other proinflammatory cytokines. Thus, IL-12 induced IFN- gamma acts in a positive feedback loop that represents an important amplifying mechanism in the inflammatory response to infections. Its role in directing development of a Th1 type immune response from naive T cells demonstrates its critical role in regulation of the immune response and strongly suggests its potential usefulness in cancer therapy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for IL-12 p70/IL-12A ELISA Kit (NBP1-92710). (Showing 1 - 1 of 1 FAQ).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: IL12A