Human IL-12 p70/IL-12A ELISA Kit (Colorimetric)

ELISA: Human IL-12 p70/IL-12A ELISA Kit (Colorimetric) [NBP1-91183] - Data for Human IL-12 (p70) ELISA Kit

Product Discontinued

Human IL-12 p70/IL-12A ELISA Kit (Colorimetric) Summary

• Description: The human IL-12 p70 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-12 p70
• Specificity: The cross reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-12p70 positive sample. There was no cross reactivity or interference detected.
• Standard Curve Range: 3.1 - 200 pg/mL
• Sensitivity: 2.1 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 4.80%
• Intra-Assay: CV% < 3.00%
• Spike Recovery: The recovery ranged from 79% to 118% with an overall mean recovery of 103%. Please see protocol for more specific Spike Recovery details.
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL12A

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human IL-12 p70/IL-12A ELISA Kit (Colorimetric)

• CLMF p35
• Cytotoxic lymphocyte maturation factor 35 kDa subunit
• IL12 p70
• IL-12 p70
• IL-12 subunit p35
• IL-12, subunit p35
• interleukin 12, p35
• interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35)
• interleukin-12 alpha chain
• interleukin-12 subunit alpha
• natural killer cell stimulatory factor 1, 35 kD subunit
• NF cell stimulatory factor chain 1
• NK cell stimulatory factor chain 1
• NKSF1
• p35

Background

Biologically active IL-12 is a disulfide-linked heterodimeric 70-kDa cytokine composed of a 35-kDa (p35) and a 40-kDa (p40) subunit. p35, a member of the IL-6 superfamily, is secreted in response to IFN gamma and agonists of TLR3, 4, or 7. However, p35 expression has been shown to be inhibited by Th2 cytokines and expressed at much lower levels than p40. Expression of p40 is regulated independently of p35. Moreover, p40 has been shown to be secreted as either a monomer or homodimer. Although each subunit alone does not possess IL-12 bioactivity, the p40 homodimer can bind to the IL-12 receptor and act as an antagonist to IL-12 p70. The receptor for this cytokine is composed of two subunits, IL-12R beta 1 and IL-12R beta 2, the latter of which is the signaling component of the receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for IL-12 p70/IL-12A ELISA Kit (NBP1-91183)(1)

Publication using NBP1-91183

• Zhang R, Yuan F, Shu Y Personalized neoantigen-pulsed dendritic cell vaccines show superior immunogenicity to neoantigen-adjuvant vaccines in mouse tumor models Cancer Immunol. Immunother. 2019-12-05 [PMID: 31807878] (ELISA, Mouse)

FAQs for IL-12 p70/IL-12A ELISA Kit (NBP1-91183). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL12A