Human IL-12 p70/IL-12A ELISA Kit (Colorimetric)

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Human IL-12 p70/IL-12A ELISA Kit (Colorimetric) [NBP1-91183] - Data for Human IL-12 (p70) ELISA Kit

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Standard Curve Range
3.1 - 200 pg/mL
Sensitivity
2.1 pg/mL

Order Details

Human IL-12 p70/IL-12A ELISA Kit (Colorimetric) Summary

Specificity
The cross reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-12p70 positive sample. There was no cross reactivity or interference detected.
Standard Curve Range
3.1 - 200 pg/mL
Sensitivity
2.1 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
4.80%
Intra-Assay
3.00%
Spike Recovery
The recovery ranged from 79% to 118% with an overall mean recovery of 103%. Please see protocol for more specific Spike Recovery details.
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
IL12A

Applications/Dilutions

Application Notes
The human IL-12 p70 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-12 p70

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Substrate Solution
  2. Streptavidin-HRP
  3. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  4. Stop Solution (1M Phosphoric acid)
  5. Sample Diluent
  6. Human IL-12p70 Standard lyophilized, 400 pg/ml upon reconstitution
  7. Adhesive Films
  8. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-12p70
  9. Amplification Reagent II
  10. Amplification Diluent Concentrate (2x)
  11. Amplification Reagent I*
  12. Biotin-Conjugate anti-human IL-12p70 monoclonal antibody
  13. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  14. * reagent contains ethyl alcohol

Alternate Names for Human IL-12 p70/IL-12A ELISA Kit (Colorimetric)

  • CLMF p35
  • Cytotoxic lymphocyte maturation factor 35 kDa subunit
  • IL12 p70
  • IL-12 p70
  • IL-12 subunit p35
  • IL-12, subunit p35
  • interleukin 12, p35
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35)
  • interleukin-12 alpha chain
  • interleukin-12 subunit alpha
  • natural killer cell stimulatory factor 1, 35 kD subunit
  • NF cell stimulatory factor chain 1
  • NK cell stimulatory factor chain 1
  • NKSF1
  • p35

Background

Biologically active IL-12 is a disulfide-linked heterodimeric 70-kDa cytokine composed of a 35-kDa (p35) and a 40-kDa (p40) subunit. p35, a member of the IL-6 superfamily, is secreted in response to IFN gamma and agonists of TLR3, 4, or 7. However, p35 expression has been shown to be inhibited by Th2 cytokines and expressed at much lower levels than p40. Expression of p40 is regulated independently of p35. Moreover, p40 has been shown to be secreted as either a monomer or homodimer. Although each subunit alone does not possess IL-12 bioactivity, the p40 homodimer can bind to the IL-12 receptor and act as an antagonist to IL-12 p70. The receptor for this cytokine is composed of two subunits, IL-12R beta 1 and IL-12R beta 2, the latter of which is the signaling component of the receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol IL12A
Entrez