IL-12 p70/IL-12A Antibody (24945) [Unconjugated]

IL‑12 p70 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL‑12 p70 Monoclonal Antibody (Catalog # MAB611) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: ELISA(Cap), ICC/IF
• Clone: 24945
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IL-12 p70/IL-12A Antibody (24945) [Unconjugated] Summary

• Immunogen: Sf 21-derived recombinant human IL-12 heterodimer
  Arg23-Ser219 of p35; Ile23-Ser328 of p40
  Accession # P29459 (p35) & P29460 (p40)
• Specificity: Detects human IL-12 p70 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human (rh) IL-12 p40, rhIL-12 p35, rhIL-23 or recombinant mouse IL-12 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL12A

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Immunocytochemistry 8-25 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Detection: Human IL-12 Biotinylated Antibody (Catalog number BAF219)
  Standard: Recombinant Human IL-12 (Catalog number 219-IL)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-12 p70/IL-12A Antibody (24945) [Unconjugated]

• CLMF p35
• Cytotoxic lymphocyte maturation factor 35 kDa subunit
• IL12 p70
• IL-12 p70
• IL-12 subunit p35
• IL-12, subunit p35
• interleukin 12, p35
• interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35)
• interleukin-12 alpha chain
• interleukin-12 subunit alpha
• natural killer cell stimulatory factor 1, 35 kD subunit
• NF cell stimulatory factor chain 1
• NK cell stimulatory factor chain 1
• NKSF1
• p35

Background

Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotrophic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and murine IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system. IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and natural killer (NK) cells. These effects include inducing production of IFN-gamma and TNF by resting and activated T and NK cells, synergizing with other IFN-gamma  inducers at both the transcriptional and post-transcriptional levels. This interaction induces IFN-gamma  gene expression, enhancing the cytotoxic activity of resting NK and T cells, inducing and synergizing with IL-2 in the generation of lymphokine-activated killer (LAK) cells, acting as a co-mitogen to stimulate proliferation of resting T cells, and inducing proliferation of activated T and NK cells. Current evidence indicates that IL-12, produced by macrophages in response to infectious agents, is a central mediator of the cell-mediated immune response by its actions on the development, proliferation, and activities of TH1 cells. In its role as the initiator of cell-mediated immunity, it has been suggested that IL-12 has therapeutic potential as a stimulator of cell-mediated immune responses to microbial pathogens, metastatic cancers, and viral infections such as AIDS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-12 p70/IL-12A Antibody (MAB611)(8)

Publications using MAB611

• Histamine regulates actin cytoskeleton in human Toll like receptor 4 activated monocyte derived dendritic cells tuning CD4+ T lymphocyte response J Biol Chem, 2016;0(0):. 2016 [PMID: 27226579] (IHC, Human)
• Perrot I, Deauvieau F, Massacrier C TLR3 and Rig-like receptor on myeloid dendritic cells and Rig-like receptor on human NK cells are both mandatory for production of IFN-gamma in response to double-stranded RNA. J. Immunol., 2010;185(4):2080-8. 2010 [PMID: 20639488] (Neutralization, Mouse)
• Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo. AIDS Res. Hum. Retroviruses, 2008;24(3):453-62. 2008 [PMID: 18327985] (Luminex Development, Human)
• Pfleger C, Schloot N, ter Veld F Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays. J. Immunol. Methods, 2007;329(1):214-8. 2007 [PMID: 17964594] (Luminex Development, Human)
• Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1. AIDS, 2007;21(10):1263-72. 2007 [PMID: 17545702] (Luminex Development, Human)
• Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Blood, 2007;109(10):4272-9. 2007 [PMID: 17289812] (Luminex Development, Human)
• Piskin G, Tursen U, Sylva-Steenland RM, Bos JD, Teunissen MB Clinical improvement in chronic plaque-type psoriasis lesions after narrow-band UVB therapy is accompanied by a decrease in the expression of IFN-gamma inducers -- IL-12, IL-18 and IL-23. Exp. Dermatol., 2004;13(12):764-72. 2004 [PMID: 15560760] (IHC, Human)
• Copeland S, Siddiqui J, Remick D Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines. J. Immunol. Methods, 2004;284(1):99-106. 2004 [PMID: 14736420] (Array Development, Human)

Bioinformatics

• Gene Symbol: IL12A
• Uniprot:
  • P29459 (p35) & P29460 (p40)()