IL-10R alpha Antibody (105653)

• Reactivity: Mu
• Applications: WB
• Clone: 105653
• Clonality: Monoclonal
• Host: Hamster
• Conjugate: Unconjugated

IL-10R alpha Antibody (105653) Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑10 R alpha
  Thr24-Asn238 ( Lys188Arg and Glu222Gly)
  Accession # Q61727
• Specificity: Detects mouse IL-10 R alpha in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) IL‑10 R alpha or rhIL-10 R beta.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Hamster
• Gene: IL10RA
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-10R alpha Antibody (105653)

• CD210 antigen
• CDw210a
• HIL-10R
• IBD28
• IL-10 R alpha
• IL-10 receptor subunit alpha
• IL10R alpha
• IL-10R subunit 1
• IL-10R1
• IL10RA
• IL-10Ra
• IL10RIL-10R subunit alpha
• interleukin 10 receptor, alpha
• interleukin-10 receptor alpha chain
• Interleukin-10 receptor subunit 1
• interleukin-10 receptor subunit alpha

Background

IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), is a potent immunosuppressant of macrophage functions. IL-10 is also a pleiotropic cytokine with multiple immunostimulatory as well as immunosuppressive effects on a variety of other cell types. IL-10 binds specifically and with high affinity to cell-surface receptors. Mouse and human cDNA clones encoding the ligand-binding IL-10 receptor (IL-10 R) have been isolated. The IL-10 R mRNA has been detected in all cell types that are known to respond to IL-10. Human and mouse IL-10 receptors are structurally related to the IFN-gamma receptor. These receptors are members of the class II subgroup of the cytokine receptor superfamily. The deduced amino acid sequence of human IL-10 R is approximately 60% identical to mouse IL-10 R. Although human IL-10 has cross-species activities and is active on mouse cells, mouse IL-10 is species-specific in its actions and does not bind to the human IL-10 receptor. The human IL-10 R gene has been mapped to chromosome 11q23.3. Recombinant IL-10 soluble receptor, consisting of the extracellular domain of IL-10 R, binds IL-10 with high affinity in solution and is a potent IL-10 antagonist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-10R alpha Antibody (MAB474)(1)

Publication using MAB474

• Rosini L, Matlack R, Taylor J, Howell KF, Yeh K, Pennello A, Riggs JE Nonlymphoid peritoneal cells suppress the T cell response to Mls. Immunobiology, 2004;209(8):575-84. 2004 [PMID: 15638126] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: IL10RA
• Uniprot:
  • Q61727()