IL-1 RII Antibody (34141) [Phycoerythrin]

Human peripheral blood mononuclear cells (PBMCs) were stained with either (A) Mouse Anti-Human IL-1 RII PE-conjugated Monoclonal Antibody (Catalog # FAB663P) or (B) Mouse IgG1Phycoerythrin Isotype Control (IC002P) and Mouse anti-Human CD14 APC-conjugated Monoclonal Antibody (FAB3832A). View our protocol for Staining Membrane-associated Proteins.

Human peripheral blood mononuclear cells (PBMCs), stimulated to induce Regulatory T Cells (Tregs), were treated with 10 ug/mL Anti-CD3, 5 ug/mL Anti-CD28, 10 ng/mL Recombinant Human TGF-beta 1 (Catalog # 240-B), and 20 ng/mL Recombinant Human IL-2 (202-IL) for 48 hours and stained with Rat Anti-Human LRRC32/GARP APC-conjugated Monoclonal Antibody and either (A) Mouse Anti-Human IL-1 RII PE-conjugated Monoclonal Antibody (Catalog # FAB663P) or (B) Mouse IgG1Phycoerythrin Isotype Control (IC002P). Cells were gated on CD4. View our protocol for Staining Membrane-associated Proteins.

HDLM-2 cells were stained with Mouse Anti-Human IL‑1 RII PE‑conjugated Monoclonal Antibody (Catalog # FAB663P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 34141
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

IL-1 RII Antibody (34141) [Phycoerythrin] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑1 RII
  Phe14-Glu343 (Ser56Gly and Glu297Gly)
  Accession # P27930
• Specificity: Detects human IL-1 RII in direct ELSAs and Western blots. When used in combination with the biotinylated human IL-1 RII affinity purified polyclonal detection antibody (Catalog # BAF263) in sandwich ELISAs, no significant cross-reactivity or interference was observed with recombinant human (rh) IL-1ra, rhIL-1 RI, recombinant mouse IL-1ra, or recombinant rat IL-1ra.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL1R2
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 RII Antibody (34141) [Phycoerythrin]

• beta
• CD121b antigen
• CD121b
• IL-1 R beta
• IL-1 RII
• IL1R2
• IL1RBCD121 antigen-like family member B
• IL-1R-beta
• IL1RII
• IL-1RII
• IL-1RT2
• IL-1RT-2
• interleukin 1 receptor, type II
• Interleukin-1 receptor beta
• MGC47725

Background

Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes, and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence similarity in their extracellular domains. The two receptor types do not heterodimerize in a receptor complex. An IL-1 receptor accessory protein that can heterodimerize with the type I receptor in the presence of IL-1 alpha or IL-1 beta , but not IL-1ra, was identified (1). This type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling type I receptor complex. Recombinant IL-1 soluble receptor type II is a potent antagonist of IL-1 action.

1. Greenfeder, S. et al. (1995) J. Biol. Chem. 270:13757.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-1 RII Antibody (FAB663P)(4)

Publications using FAB663P

• J. Agustin Cruz, Chaitanya S. Mokashi, Gabriel J. Kowalczyk, Yue Guo, Qiuhong Zhang, Sanjana Gupta et al. A variable-gain stochastic pooling motif mediates information transfer from receptor assemblies into NF-kappa B Science Advances 7/23/2021 [PMID: 34301608]
• Karoline Walscheid Phenotypic changes of peripheral blood mononuclear cells upon corticosteroid treatment in idiopathic intermediate uveitis Clin. Immunol, 2016-10-28;0(0):. 2016-10-28 [PMID: 27989897] (Flow Cytometry, Human)
• Mattiola I, Pesant M, Tentorio P, Molgora M, Marcenaro E, Lugli E, Locati M, Mavilio D Priming of Human Resting NK Cells by Autologous M1 Macrophages via the Engagement of IL-1beta, IFN-beta, and IL-15 Pathways. J Immunol, 2015-08-14;195(6):2818-28. 2015-08-14 [PMID: 26276870] (Flow Cytometry, Human)
• F F Vasilyev, A N Silkov, S V Sennikov Relationship between interleukin-1 type 1 and 2 receptor gene polymorphisms and the expression level of membrane-bound receptors Cellular & Molecular Immunology 2015-03-01 [PMID: 24976267] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: IL1R2
• Uniprot:
  • P27930()