IKK epsilon/IKBKE Antibody

Western Blot: IKK epsilon/IKBKE Antibody [NB100-56329] - Analysis of IKKe in Daudi lysate using IKK epsilon/IKBKE antibody at 2 ug/ml.

Product Discontinued

IKK epsilon/IKBKE Antibody Summary

• Immunogen: This antibody was raised against synthetic peptides corresponding to amino acids 175-188 (GYNEEQIHKLDKVN), 526-540 (ELVKSRDQVHEDRSIC), and 567-580 (GTEEYLHPDMYERAC) of human IKKe protein.
• Specificity: An 80 kDa band is observed. The has tested negative on mouse NIH 3T3 cells. Based on the sequence similarity, it may cross react with TBK1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IKBKE
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/ml

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.2% gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for IKK epsilon/IKBKE Antibody

• EC 2.7.11
• EC 2.7.11.10
• I-kappa-B kinase epsilon
• IKBKE
• IKK epsilon
• IKK-E
• IKKEMGC125297
• IKK-epsilon
• IKKI
• IKK-I
• IKK-iMGC125295
• Inducible I kappa-B kinase
• inducible IkappaB kinase
• inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon
• inhibitor of nuclear factor kappa-B kinase subunit epsilon
• KIAA0151IKK-related kinase epsilon
• MGC125294

Background

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-5). Using a subtractive hybridization technique, a novel kinase, IKKi/IKKe has been isolated (6). LPS increases IKKi mRNA level in mouse macrophage cell lines. This protein has significant sequence homology with kinase domains of IKK1/a and IKK-2/b. Overexpression of wild type IKKi in cells phosphorylates Ser32 and Ser36 of IkBa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IKBKE
• Entrez:
  • 9641(Human)
• Uniprot:
  • Q14164()