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IKK epsilon/IKBKE Antibody (JU06-72)

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Western Blot: IKK epsilon/IKBKE Antibody (JU06-72) [NBP2-75537] - Analysis of IKBKE on different cell lysates using anti-IKBKE antibody at 1/500 dilution.Positive control:Lane 1: Hela Lane 2: RajiLane 3: MCF-7 Lane 4: Jurkat
Western Blot: IKK epsilon/IKBKE Antibody (JU06-72) [NBP2-75537] - Analysis of IKBKE on different cell lysates using anti-IKBKE antibody at 1/500 dilution.Positive control:Lane 1: Hela Lane 2: RajiLane 3: MCF-7 ...read more
Immunocytochemistry/ Immunofluorescence: IKK epsilon/IKBKE Antibody (JU06-72) [NBP2-75537] - Staining IKBKE in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunohistochemistry-Paraffin: IKK epsilon/IKBKE Antibody (JU06-72) [NBP2-75537] - Analysis of paraffin-embedded human kidney tissue using anti-IKBKE antibody. Counter stained with hematoxylin.
Flow Cytometry: IKK epsilon/IKBKE Antibody (JU06-72) [NBP2-75537] - Analysis of Jurkat cells with IKBKE antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary ...read more
Immunocytochemistry/ Immunofluorescence: IKK epsilon/IKBKE Antibody (JU06-72) [NBP2-75537] - Staining IKBKE in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunocytochemistry/ Immunofluorescence: IKK epsilon/IKBKE Antibody (JU06-72) [NBP2-75537] - Staining IKBKE in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
JU06-72
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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IKK epsilon/IKBKE Antibody (JU06-72) Summary

Immunogen
Recombinant protein within human IKBKE aa 1-200
Localization
Cytoplasm, Nucleus.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
IKBKE
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin
Theoretical MW
71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40%Glycerol
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for IKK epsilon/IKBKE Antibody (JU06-72)

  • EC 2.7.11
  • EC 2.7.11.10
  • I-kappa-B kinase epsilon
  • IKBKE
  • IKK epsilon
  • IKK-E
  • IKKEMGC125297
  • IKK-epsilon
  • IKKI
  • IKK-I
  • IKK-iMGC125295
  • Inducible I kappa-B kinase
  • inducible IkappaB kinase
  • inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon
  • inhibitor of nuclear factor kappa-B kinase subunit epsilon
  • KIAA0151IKK-related kinase epsilon
  • MGC125294

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: IKK beta Antibody (10AG2) [NB100-56509] - Human gall bladder probed with IKKbeta antibody at 5 ug/ml. Human tissue TMA was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
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Immunohistochemistry: HGK/MAP4K4 Antibody [NBP1-58853] - Liver
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Western blot shows recombinant human JNK1, JNK2, and JNK3 (1 ng/lane). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for JNK1 was detected at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
(2 Reviews)
(3 Publications)
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TANK Antibody
NB100-56144
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Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP
IKK epsilon/IKBKE
Antibody (JU06-72)
NBP2-75537
IKK epsilon/IKBKE Antibody (JU06-72)
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P

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Bioinformatics

Gene Symbol IKBKE