IKK epsilon/IKBKE Antibody (JU06-72)

Western Blot: IKK epsilon/IKBKE Antibody (JU06-72) [NBP2-75537] - Analysis of IKBKE on different cell lysates using anti-IKBKE antibody at 1/500 dilution.Positive control:Lane 1: Hela Lane 2: RajiLane 3: MCF-7 Lane 4: Jurkat

Immunocytochemistry/Immunofluorescence: IKK epsilon/IKBKE Antibody (JU06-72) [NBP2-75537] - Staining IKBKE in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: IKK epsilon/IKBKE Antibody (JU06-72) [NBP2-75537] - Analysis of paraffin-embedded human kidney tissue using anti-IKBKE antibody. Counter stained with hematoxylin.

Flow Cytometry: IKK epsilon/IKBKE Antibody (JU06-72) [NBP2-75537] - Analysis of Jurkat cells with IKBKE antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: IKK epsilon/IKBKE Antibody (JU06-72) [NBP2-75537] - Staining IKBKE in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: IKK epsilon/IKBKE Antibody (JU06-72) [NBP2-75537] - Staining IKBKE in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

IKK epsilon/IKBKE Antibody (JU06-72) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human IKK epsilon/IKBKE aa 26-75 / 716. (SwissProt: Q14164 Human)
• Localization: Cytoplasm, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IKBKE
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for IKK epsilon/IKBKE Antibody (JU06-72)

• EC 2.7.11
• EC 2.7.11.10
• I-kappa-B kinase epsilon
• IKBKE
• IKK epsilon
• IKK-E
• IKKEMGC125297
• IKK-epsilon
• IKKI
• IKK-I
• IKK-iMGC125295
• Inducible I kappa-B kinase
• inducible IkappaB kinase
• inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon
• inhibitor of nuclear factor kappa-B kinase subunit epsilon
• KIAA0151IKK-related kinase epsilon
• MGC125294

Background

Nuclear Factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during the activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cytoplasm of the cell, which inhibit NF-kB activity. IkB proteins are phosphorylated by an IkB kinase complex consisting of at least three proteins, IKKa, IKKb, and IKKg. Isolated from a cDNA library of LPS-stimulated mouse macrophage cells, a novel molecule in the IKK complex has been recently identified and designated IKKi and/or IKKe. IKKe is required for the activation of NF-kB by mitogens and T cell receptors but not by TNFa or IL-1. LPS increases the IKKe mRNA level in mouse macrophage cell lines. This protein has significant sequence homology with kinase domains of IKKa and IKKb. Overexpression of wild type IKKe in cells phosphorylates Ser32 and Ser36 of IkBa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IKBKE