IKK beta Antibody (3C12) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: IKK beta Antibody (3C12) [H00003551-M08] - Analysis of monoclonal antibody to IKBKB on HeLa cell. Antibody concentration 10 ug/ml

Sandwich ELISA: IKK beta Antibody (3C12) [H00003551-M08] - Detection limit for recombinant GST tagged IKBKB is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 3C12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

IKK beta Antibody (3C12) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: IKBKB (AAH06231, 3 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. WSPSLTTQTCGAWEMKERLGTGGFGNVIRWHNQETGEQIAIKQCRQELSPRNRERWCLEIQIMRRLTHPNVVAARDVPEGMQNLAPNDLPLLAMEYCQGGDLRKYLNQFENCCGLREG
• Specificity: IKBKB (3C12)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IKBKB
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for IKK beta Antibody (3C12) - Azide and BSA Free

• EC 2.7.11
• EC 2.7.11.10
• I-kappa-B kinase 2
• I-kappa-B-kinase beta
• IkBKB
• IKK beta
• IKK2
• IKK2FLJ38368
• IKK-B
• IKK-betaFLJ36218
• IKKBFLJ40509
• inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
• inhibitor of nuclear factor kappa-B kinase subunit beta
• MGC131801
• NFKBIKB
• NFKBIKBFLJ33771
• Nuclear factor NF-kappa-B inhibitor kinase beta

Background

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IKBKB
• Entrez:
  • 3551(Human)
• OMIM:
  • 603258(Human)
• Uniprot:
  • AAH06231(Human)