IkB-beta Antibody (1G6)

Images

 
Western Blot: IkB-beta Antibody (1G6) [H00004793-M06] - Analysis of NFKBIB expression in transfected 293T cell line by NFKBIB monoclonal antibody (M06), clone 1G6. Lane 1: NFKBIB transfected lysatE (37.8 KDa). Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: IkB-beta Antibody (1G6) [H00004793-M06] - Analysis of monoclonal antibody to NFKBIB on HeLa cell. Antibody concentration 40 ug/ml
Sandwich ELISA: IkB-beta Antibody (1G6) [H00004793-M06] - Detection limit for recombinant GST tagged NFKBIB is 0.1 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
1G6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

IkB-beta Antibody (1G6) Summary

Immunogen
NFKBIB (AAH15528, 1 a.a. - 356 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAGVACLGKAADADEWCDSGLGSLGPDAAAPGGPGLGAELGPGLSWAPLVFGYVTEDGDTALHLAVIHQHEPFLDFLLGFSAGTEYMDLQNDLGQTALHLAAILGETSTVEKLYAAGAGLCVAERRGHTALHLACRVGAHACARALLQPRPRRPREAPDTYLAQGPDRTPDTNHTPVALYPDSDLEKEEEESEEDWKLQLEAENYEGHTPLHVAVIHKDVEMVRLLRDAGADLDKPEPTCGRSPLHLAVEAQAADVLELLLRAGANPAARMYGGRTPLGSAMLRPNPILARLLRAHGAPEPEGEDEKSGPCSSSSDSDSGDEGDEYDDIVVHSSRSQTRLPPTPASKPLPDDPRPV
Specificity
NFKBIB - nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta (1G6)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NFKBIB
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for IkB-beta Antibody (1G6)

  • beta
  • IkappaBbeta
  • I-kappa-B-beta
  • ikB-B
  • IkBbeta
  • IkB-beta
  • IKBBikB-beta
  • NF-kappa-B inhibitor beta
  • NF-kappa-BIB
  • NFKBIB
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor
  • Thyroid receptor-interacting protein 9
  • TR-interacting protein 9
  • TRIP-9
  • TRIP9ikappaBbeta

Background

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664 or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NFKBIB
Entrez
Uniprot