IkB-alpha [p Ser32, p Ser36, p Ser36, p Ser32] Antibody

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA) for 5 minutes. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human Phospho-IkB-a (S32/S36) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4809) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-IkB-a (S32/S36) was detected at approximately 44 kDa (as indicated). For additional reference, duplicate lysates were probed with 0.1 µg/mL Human IkB-a Monoclonal Antibody (lower panel, Catalog # MAB4299). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Product Discontinued

IkB-alpha [p Ser32, p Ser36, p Ser36, p Ser32] Antibody Summary

• Immunogen: Phosphopeptide containing human IkB-alpha S32/36 sites
• Modification: p Ser36, p Ser32, p Ser32, p Ser36
• Specificity: Detects human IkB-alpha when phosphorylated at S32/36.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NFKBIA
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IkB-alpha [p Ser32, p Ser36, p Ser36, p Ser32] Antibody

• alpha
• I-kappa-B-alpha
• IkappaBalphaNF-kappa-B inhibitor alpha
• IKBAikB-alpha
• IkBalpha
• IkB-alpha
• MAD3
• MAD-3
• Major histocompatibility complex enhancer-binding protein MAD3
• NFKBI
• NFKBIA
• nuclear factor of kappa light chain gene enhancer in B-cells
• nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor

Background

NF kappa B inhibitor alpha (IkB‑ alpha ) inhibits the NF kappa B complex by binding and sequestering it in the cytoplasm. Upon stimulation, IkB‑ alpha is phosphorylated on serine residues 32 and 36 marking it for degradation by the ubiquitin pathway. Following IkB‑ alpha degradation, the NF kappa B complex is able to translocate to the nucleus and activate transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IkB-alpha Antibody (AF4809)(4)

Publications using AF4809

• CM Smolko, KA Janes An ultrasensitive fiveplex activity assay for cellular kinases Sci Rep, 2019-12-19;9(1):19409. 2019-12-19 [PMID: 31857650] (Multiplex Assay, Human)
• SY Ho, WS Wu, LC Lin, YH Wu, HW Chiu, YL Yeh, BM Huang, YJ Wang Cordycepin Enhances Radiosensitivity in Oral Squamous Carcinoma Cells by Inducing Autophagy and Apoptosis Through Cell Cycle Arrest Int J Mol Sci, 2019-10-28;20(21):. 2019-10-28 [PMID: 31661901] (Western Blot, Human)
• K Seno, Y Munakata, M Sano, R Kawahara-M, H Takahashi, A Ohkuchi, H Iwata, T Kuwayama, K Shirasuna Aggregation of Human Trophoblast Cells into Three-Dimensional Culture System Enhances Anti-Inflammatory Characteristics through Cytoskeleton Regulation Int J Mol Sci, 2018-08-08;19(8):. 2018-08-08 [PMID: 30096769] (Western Blot, Human)
• Xu J, Wu F, Tian D, Wang J, Zheng Z, Xia N Open reading frame 3 of genotype 1 hepatitis E virus inhibits nuclear factor-kappaappa B signaling induced by tumor necrosis factor-alpha in human A549 lung epithelial cells. PLoS ONE, 2014-06-24;9(6):e100787. 2014-06-24 [PMID: 24959724] (Western Blot, Human)

Bioinformatics

• Gene Symbol: NFKBIA