IkB-alpha [p Ser32, p Ser36] Antibody (39A1413)


Western Blot: IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) [NB100-56724] - Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal) - a Calpain inhibitor and also proteasome inhibitor ...read more
Immunoprecipitation: IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) [NB100-56724] - Analysis using the Biotin conjugate of NB100-56724. Detection of Jurkat cells were treated for 30 min with 100 ug/ml ALLN ...read more

Product Details

Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, IP
1.0 mg/ml

IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) Summary

This monoclonal antibody was raised against a synthetic peptide containing phosphorylated serines at amino acid residues 32 and 36 of human IkBa.
p Ser32, p Ser36
IkBa (39A1413 ) [Ser32/Ser36]
IgG1 Kappa
Protein G purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein G purified


  • Western Blot 1-2 ug/ml
  • Immunoprecipitation 1-2 ug/ml
Read Publications using
NB100-56724 in the following applications:

  • WB
    5 publications

Alternate Names for IkB-alpha [p Ser32, p Ser36] Antibody (39A1413)

  • alpha
  • I-kappa-B-alpha
  • IkappaBalphaNF-kappa-B inhibitor alpha
  • IKBAikB-alpha
  • IkBalpha
  • IkB-alpha
  • MAD3
  • MAD-3
  • Major histocompatibility complex enhancer-binding protein MAD3
  • nuclear factor of kappa light chain gene enhancer in B-cells
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor


NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. External stimuli such as tumor necrosis factor or other cytokines initiates a signal transduction cascade that leads to the activation of IkB-kinase complex that specifically phosphorylates IkBa on Serine-32 and Serine-36. Phosphorylation of these sites leads to ubiquitination of IkBa and subsequent degradation by the 26 S proteasome. Degradation of IkBa results in unmasking of the nuclear localization signal of NF-kB dimers, which subsequently translocates to the nucleus and activates target genes (7,8). Six members of IkB family members have been identified. One of the first gene induced following NF-kB activation is IkBa.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IkB-alpha Antibody (NB100-56724)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.

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Showing Publications 1 - 9 of 9.
Publications using NB100-56724 Applications Species
Samanta D, Gilkes D, Chaturvedi P, Xiang L, Semenza G. Hypoxia-inducible factors are required for chemotherapy resistance of breast cancer stem cells. Proc Natl Acad Sci U S A. 2014 Dec 1 [PMID:25453096] (WB, Human) WB Human
Das U, Manna K, Sinha M et al. Role of ferulic Acid in the amelioration of ionizing radiation induced inflammation: a murine model. PLoS ONE. 2014 May 23 [PMID:24854039] (WB, Mouse) WB Mouse
Shakibaei Mehdi, Allaway David, Nebrich Simone, Mobasheri Ali. Botanical Extracts from Rosehip (Rosa canina), Willow Bark (Salix alba), and Nettle Leaf (Urtica dioica) Suppress IL-1beta-Induced NF-kappaB Activation in Canine Articular Chondrocytes. Evid Based Complement Alternat Med. 2012 [PMID:22474508]
Li Q, Guo Y, Tan W et al. Cardioprotection afforded by inducible nitric oxide synthase gene therapy is mediated by cyclooxygenase-2 via a nuclear factor-kappaB dependent pathway. Circulation. 2007 Oct 2 [PMID:17785622] (WB, Mouse) WB Mouse
Yang M, Hase H, Legarda-Addison D et al. B cell maturation antigen, the receptor for a proliferation-inducing ligand and B cell-activating factor of the TNF family, induces antigen presentation in B cells. J Immunol. 2005 Sep 1 [PMID:16116167] (WB) WB
Jazirehi AR, Huerta-Yepez S, Cheng G et al. Rituximab (chimeric anti-CD20 monoclonal antibody) inhibits the constitutive nuclear factor-{kappa}B signaling pathway in non-Hodgkin's lymphoma B-cell lines: role in sensitization to chemotherapeutic drug-induced apoptosis. Cancer Res. 2005 Jan 1 [PMID:15665303] (WB) WB
Lotz Sonja, Aga Eresso, Wilde Inga et al. Highly purified lipoteichoic acid activates neutrophil granulocytes and delays their spontaneous apoptosis via CD14 and TLR2. J Leukoc Biol. 2004 Mar [PMID:14673018]
Ikezoe T, Yang Y, Heber D et al. PC-SPES: a potent inhibitor of nuclear factor-kappa B rescues mice from lipopolysaccharide-induced septic shock. Mol Pharmacol. 2003 Dec [PMID:14645683]
Han HS, Karabiyikoglu M, Kelly S et al. Mild hypothermia inhibits nuclear factor-kappaB translocation in experimental stroke. J Cereb Blood Flow Metab. 2003 May [PMID:12771574]

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Product General Protocols

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WB Video Protocol

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56724AF405
Alexa Fluor 488 Labeled NB100-56724AF488
Alexa Fluor 647 Labeled NB100-56724AF647
Alexa Fluor 700 Labeled NB100-56724AF700
Allophycocyanin Labeled NB100-56724APC
Biotin Labeled NB100-56065
DyLight 350 Labeled NB100-56724UV
DyLight 405 Labeled NB100-56724V
DyLight 405LS Labeled NB100-56724V3
DyLight 488 Labeled NB100-56724G
DyLight 550 Labeled NB100-56724R
DyLight 650 Labeled NB100-56724C
DyLight 680 Labeled NB100-56724FR
DyLight 755 Labeled NB100-56724IR
HRP Labeled NB100-56724H
PE Labeled NB100-56724PE
PerCP Labeled NB100-56724PCP

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Blogs on IkB-alpha.

Inhibitor kappa B-alpha (IkappaB-alpha)
The transcription factor nuclear factor kappa beta (NFkB) is highly regulated by triggers such as stress, free-radicals, UV light, and hypoxia. NFkB is one of the fastest responding transcription factors in humans. The NFKB signaling pathway is ess...  Read full blog post.

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Gene Symbol NFKBIA

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