IkB-alpha [p Ser32, p Ser36] Antibody (39A1413)


Biological Strategies: Western Blot: IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) [NB100-56724] - Analysis using the Biotin conjugate of NB100-56724. Detection of Jurkat cells were treated for 30 min with 100 ...read more
Biological Strategies: Western Blot: IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) [NB100-56724] - Jurkat cells were treated for 30 min with 100 ug/ml ALLN (N-Acetyl-Leu-Leu-Norleucinal) - a Calpain inhibitor ...read more

Product Details

Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, IP
1.0 mg/ml
Validated by:

Biological Strategies


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IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) Summary

This monoclonal antibody was raised against a synthetic peptide containing phosphorylated serines at amino acid residues 32 and 36 of human IkBa.
p Ser32, p Ser36
IkBa (39A1413 ) [Ser32/Ser36]
IgG1 Kappa
Protein G purified
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  • Western Blot 1-2 ug/ml
  • Immunoprecipitation 1-2 ug/ml
Read Publications using
NB100-56724 in the following applications:

  • WB
    8 publications

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for IkB-alpha [p Ser32, p Ser36] Antibody (39A1413)

  • alpha
  • I-kappa-B-alpha
  • IkappaBalphaNF-kappa-B inhibitor alpha
  • IKBAikB-alpha
  • IkBalpha
  • IkB-alpha
  • MAD3
  • MAD-3
  • Major histocompatibility complex enhancer-binding protein MAD3
  • nuclear factor of kappa light chain gene enhancer in B-cells
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor


NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. External stimuli such as tumor necrosis factor or other cytokines initiates a signal transduction cascade that leads to the activation of IkB-kinase complex that specifically phosphorylates IkBa on Serine-32 and Serine-36. Phosphorylation of these sites leads to ubiquitination of IkBa and subsequent degradation by the 26 S proteasome. Degradation of IkBa results in unmasking of the nuclear localization signal of NF-kB dimers, which subsequently translocates to the nucleus and activates target genes (7,8). Six members of IkB family members have been identified. One of the first gene induced following NF-kB activation is IkBa.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IkB-alpha Antibody (NB100-56724)(12)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 1 application: WB.

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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB100-56724 Applications Species
Wen ZS, Tang Z, Gu LX et al. Immunomodulatory effect of low molecular-weight seleno-aminopolysaccharide on immunosuppressive mice. Int. J. Biol. Macromol. Oct 17 2018 [PMID: 30342138] (WB, Mouse) WB Mouse
Kanno Y, Maruyama C, Matsuda A, Ishisaki A. uPA-derived peptide, A6 is involved in the suppression of lipopolysaccaride-promoted inflammatory osteoclastogenesis and the resultant bone loss. Immun Inflamm Dis May 11 2017 [PMID: 28493442] (WB, Mouse) WB Mouse
Gu LX, Wen ZS, Xiang XW et al. Immunomodulatory effect of low molecular-weight seleno-aminopolysaccharides in intestinal epithelial cells. Int. J. Biol. Macromol. Mar 4 2017 [PMID: 28267616] (WB, Porcine) WB Porcine
Samanta D, Gilkes D, Chaturvedi P, Xiang L, Semenza G. Hypoxia-inducible factors are required for chemotherapy resistance of breast cancer stem cells. Proc Natl Acad Sci U S A. 2014 Dec 1 [PMID: 25453096] (WB, Human) WB Human
Das U, Manna K, Sinha M et al. Role of ferulic Acid in the amelioration of ionizing radiation induced inflammation: a murine model. PLoS ONE. 2014 May 23 [PMID: 24854039] (WB, Mouse)

Liver, Fig 8.
WB Mouse
Shakibaei Mehdi, Allaway David, Nebrich Simone, Mobasheri Ali. Botanical Extracts from Rosehip (Rosa canina), Willow Bark (Salix alba), and Nettle Leaf (Urtica dioica) Suppress IL-1beta-Induced NF-kappaB Activation in Canine Articular Chondrocytes. Evid Based Complement Alternat Med. 2012 [PMID: 22474508]
Li Q, Guo Y, Tan W et al. Cardioprotection afforded by inducible nitric oxide synthase gene therapy is mediated by cyclooxygenase-2 via a nuclear factor-kappaB dependent pathway. Circulation. 2007 Oct 2 [PMID: 17785622] (WB, Mouse)

WB (WT, COX-2-/-, IkBa (Ser 32A,Ser 36A) Tg mice injected with iNOS gene), Fig. 5.
WB Mouse
Yang M, Hase H, Legarda-Addison D et al. B cell maturation antigen, the receptor for a proliferation-inducing ligand and B cell-activating factor of the TNF family, induces antigen presentation in B cells. J Immunol. 2005 Sep 1 [PMID: 16116167] (WB)

Antibodies cited [WB]: 1. IkBa Ser 32/Ser 36 [IMG-156A], (A20 B cells stimulated with APRIL), Fig. 2c 2. IkBa [IMG-127A], (A20 cells stimulated with anti-TNFR2), Fig. 6a
Jazirehi AR, Huerta-Yepez S, Cheng G et al. Rituximab (chimeric anti-CD20 monoclonal antibody) inhibits the constitutive nuclear factor-{kappa}B signaling pathway in non-Hodgkin's lymphoma B-cell lines: role in sensitization to chemotherapeutic drug-induced apoptosis. Cancer Res. 2005 Jan 1 [PMID: 15665303] (WB)

WB (Ramos and Daudi cells growing in RPMI 1640 and 1% fetal bovine serum and rituximab), Fig. 4a
Lotz Sonja, Aga Eresso, Wilde Inga et al. Highly purified lipoteichoic acid activates neutrophil granulocytes and delays their spontaneous apoptosis via CD14 and TLR2. J Leukoc Biol. 2004 Mar [PMID: 14673018]
Show All 12 Publications.

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-56724AF350
Alexa Fluor 405 NB100-56724AF405
Alexa Fluor 488 NB100-56724AF488
Alexa Fluor 532 NB100-56724AF532
Alexa Fluor 594 NB100-56724AF594
Alexa Fluor 647 NB100-56724AF647
Alexa Fluor 700 NB100-56724AF700
Alexa Fluor 750 NB100-56724AF750
Biotin NB100-56065
DyLight 350 NB100-56724UV
DyLight 405 NB100-56724V
DyLight 488 NB100-56724G
DyLight 550 NB100-56724R
DyLight 594 NB100-56724DL594
DyLight 650 NB100-56724C
DyLight 680 NB100-56724FR
DyLight 755 NB100-56724IR
FITC NB100-56724F
HRP NB100-56724H
Janelia Fluor 549 NB100-56724JF549
Janelia Fluor 646 NB100-56724JF646

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Blogs on IkB-alpha.

Sample collection from mammalian culture cells for kinomic analysis
By Jamshed Arslan Pharm.D., PhD.IntroductionKinome describes kinases, and kinomics refers to the kinase signaling. Studying the effects of reagent (exogenously applied growth factor or inhibitor) on kinase activit...  Read full blog post.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

Inhibitor kappa B-alpha (IkappaB-alpha)
The transcription factor nuclear factor kappa beta (NFkB) is highly regulated by triggers such as stress, free-radicals, UV light, and hypoxia. NFkB is one of the fastest responding transcription factors in humans. The NFKB signaling pathway is ess...  Read full blog post.

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Gene Symbol NFKBIA