| Reactivity | HuSpecies Glossary |
| Applications | WB, CLIA, ELISA, Flow, IA, ICC/IF, IHC, IM |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | PE/Cy5.5 |
| Immunogen | Purified Human IgM, Mu chain |
| Specificity | Based on Immunoelectrophoresis, this antibody reacts with heavy chains on human IgM. Cross Adsorbed: This antibody was pre-adsorbed to reduce cross-reactivity with proteins of non-related species. Minimal cross-reactivity with human IgA + IgG serum proteins. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | IGHM |
| Purity | >95%, by SDS-PAGE |
| Application Notes | Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions. |
| Storage | Store at 4C in the dark. Do not freeze. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Purity | >95%, by SDS-PAGE |
![Goat anti-Human IgM Secondary Antibody [PE/Cy5.5]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Goat anti-Human IgM Secondary Antibody [PE/Cy5.5]")
| Alkaline Phosphatase | NBP1-75018 | |
| Allophycocyanin/Cy7 | NBP1-75017APCCY7 | |
| Biotin | NBP1-75019 | |
| DyLight 350 | NBP1-75017UV | |
| DyLight 405 | NBP1-75017V | |
| DyLight 488 | NBP1-75017G | |
| DyLight 550 | NBP1-75892 | |
| DyLight 594 | NBP1-75017DL594 | |
| DyLight 650 | NBP1-75017C | |
| DyLight 680 | NBP1-75017FR | |
| DyLight 755 | NBP1-75017IR | |
| FITC | NBP1-75020 | |
| HRP | NBP1-75021 | |
| Janelia Fluor 549 | NBP1-75017JF549 | |
| Janelia Fluor 646 | NBP1-75017JF646 | |
| PE/Atto594 | NBP1-75017PEATT594 | |
| PE/Cy5.5 | NBP1-75017PECY55 | |
| PE/Cy7 | NBP1-75017PECY7 |
Diseases for IgM Antibody (NBP1-75017PECY55)Discover more about diseases related to IgM Antibody (NBP1-75017PECY55).
| Pathways for IgM Antibody (NBP1-75017PECY55)View related products by pathway.
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Research Areas for IgM Antibody (NBP1-75017PECY55)Find related products by research area.
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How to identify B cell subsets using flow cytometry By Victoria OsinskiUsing flow cytometry to identify B cell subsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified a... Read full blog post. |
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Understanding the Importance of Human IgG Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | IGHM |
![Western Blot: MLC1 Antibody [NBP1-81555] - Analysis in control (vector only transfected HEK293T lysate) and MLC1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/images2/MLC1-Antibody-Western-Blot-NBP1-81555-img0008.jpg)
![Immunohistochemistry-Paraffin: MLC1 Antibody [NBP1-81555] - Analysis in human cerebral cortex and pancreas tissues using NBP1-81555 antibody. Corresponding MLC1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/MLC1-Antibody-Immunohistochemistry-Paraffin-NBP1-81555-img0014.jpg)
![Immunohistochemistry-Paraffin: MLC1 Antibody [NBP1-81555] - Staining of human cerebellum shows moderate cytoplasmic positivity in neuropil of the molecular layer.](http://images.novusbio.com/images2/MLC1-Antibody-Immunohistochemistry-Paraffin-NBP1-81555-img0009.jpg)
![Western Blot: RUNX1T1/ETO Antibody [NBP2-55747] - H2135 and H2087 non-small cell lung cancer cells showing baseline Runx1T1 expression versus Runx1T1 stable overexpression. Image submitted by a verified customer review.](http://images.novusbio.com/images2/RUNX1T1-ETO-Antibody-Western-Blot-NBP2-55747-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: RUNX1T1/ETO Antibody [NBP2-55747] - Staining of human cell line HEK 293 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/images2/RUNX1T1-ETO-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55747-img0001.jpg)
![Western Blot: Aldo-keto Reductase 1C4/AKR1C4 Antibody (2C11) [H00001109-M01] - Aldo-keto Reductase 1C4/AKR1C4 monoclonal antibody (M01), clone 2C11 Western Blot analysis of Aldo-keto Reductase 1C4/AKR1C4 expression in MCF-7 [L046V1].](http://images.novusbio.com/images2/Aldo-keto-Reductase-1C4-AKR1C4-Antibody-(2C11)-Western-Blot-H00001109-M01-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Aldo-keto Reductase 1C4/AKR1C4 Antibody (2C11) [H00001109-M01] - Analysis of monoclonal antibody to AKR1C4 on HepG2 cell. Antibody concentration 15 ug/ml.](http://images.novusbio.com/images2/Aldo-keto-Reductase-1C4-AKR1C4-Antibody-(2C11)-Immunocytochemistry-Immunofluorescence-H00001109-M01-img0011.jpg)
![Immunohistochemistry-Paraffin: Aldo-keto Reductase 1C4/AKR1C4 Antibody (2C11) [H00001109-M01] - Analysis of monoclonal antibody to AKR1C4 on formalin-fixed paraffin-embedded human liver tissue. Antibody concentration 3 ug/ml.](http://images.novusbio.com/images2/Aldo-keto-Reductase-1C4-AKR1C4-Antibody-(2C11)-Immunohistochemistry-Paraffin-H00001109-M01-img0013.jpg)
![Western Blot: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GLB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/images2/beta-Galactosidase-1-GLB1-Antibody-(1C9)-Western-Blot-NBP2-45731-img0016.jpg)
![Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (1C9) [NBP2-45731] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/images2/beta-Galactosidase-1-GLB1-Antibody-(1C9)-Immunohistochemistry-NBP2-45731-img0013.jpg)
![Western Blot: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLB1.](http://images.novusbio.com/images2/beta-Galactosidase-1-GLB1-Antibody-(1C9)-Western-Blot-NBP2-45731-img0014.jpg)
![Western Blot: IFN-alpha 1 Antibody [NBP2-75930] - analysis of IFNA1 on mouse liver tissue lysate using anti-IFNA1 antibody at 1/100 dilution.](http://images.novusbio.com/images2/IFN-alpha-1-Antibody-Western-Blot-NBP2-75930-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: IFN-alpha 1 Antibody [NBP2-75930] - staining IFNA1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/IFN-alpha-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-75930-img0004.jpg)
![Immunohistochemistry-Paraffin: IFN-alpha 1 Antibody [NBP2-75930] - analysis of paraffin-embedded mouse brain tissue tissue using anti-IFNA1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/IFN-alpha-1-Antibody-Immunohistochemistry-Paraffin-NBP2-75930-img0007.jpg)
![Western Blot: CDR2 Antibody (33) [NBP2-10509] - Western blot analysis of CDR2 expression in 1) HeLa control cell lysate and 2) HeLa cell lysate overexpressing CDR2.](http://images.novusbio.com/images/CDR2-Antibody-(33)-Western-Blot-NBP2-10509-img0003.jpg)
![Immunohistochemistry: CDR2 Antibody (33) [NBP2-10509] - IHC staining of CDR2 in human breast cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/images2/CDR2-Antibody-(33)-Immunohistochemistry-NBP2-10509-img0004.jpg)
![Western Blot: CDR2 Antibody (33) [NBP2-10509] - Western blot analysis of CDR2 expression in 1) HeLa, 2) NTERA-2 and 3) HepG2 whole cell lysates.](http://images.novusbio.com/images/CDR2-Antibody-(33)-Western-Blot-NBP2-10509-img0002.jpg)
![Western Blot: Glutathione S Transferase kappa 1 Antibody (JE47-38) [NBP2-80461] - Western blot analysis of GSTK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1:1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5000 dilution was used for 1 hour at room temperature.Positive control:Lane 1: SK-BR-3 cell lysateLane 2: Human skin tissue lysateLane 3: Human placenta tissue lysate](http://images.novusbio.com/images2/Glutathione-S-Transferase-kappa-1-Antibody-(JE47-38)-Western-Blot-NBP2-80461-img0001.jpg)
![Immunohistochemistry-Paraffin: Glutathione S Transferase kappa 1 Antibody (JE47-38) [NBP2-80461] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-GSTK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/images2/Glutathione-S-Transferase-kappa-1-Antibody-(JE47-38)-Immunohistochemistry-Paraffin-NBP2-80461-img0005.jpg)
![Flow Cytometry: Glutathione S Transferase kappa 1 Antibody (JE47-38) [NBP2-80461] - Flow cytometric analysis of GSTK1 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (1:100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/images2/Glutathione-S-Transferase-kappa-1-Antibody-(JE47-38)-Flow-Cytometry-NBP2-80461-img0006.jpg)
![Immunohistochemistry-Paraffin: SLCO6A1 Antibody [NBP2-49118] - Staining of human testis shows high expression.](http://images.novusbio.com/images2/SLCO6A1-Antibody-Immunohistochemistry-Paraffin-NBP2-49118-img0004.jpg)
![Immunohistochemistry-Paraffin: SLCO6A1 Antibody [NBP2-49118] - Staining of human endometrium shows low expression as expected.](http://images.novusbio.com/images2/SLCO6A1-Antibody-Immunohistochemistry-Paraffin-NBP2-49118-img0003.jpg)
![Immunohistochemistry-Paraffin: SLCO6A1 Antibody [NBP2-49118] - Staining in human testis and endometrium tissues using anti-SLCO6A1 antibody. Corresponding SLCO6A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/SLCO6A1 Antibody-Immunohistochemistry-Paraffin-NBP2-49118-img0005.jpg)
![Western Blot: MKP-1/DUSP1 Antibody (JJ0930) [NBP2-67909] - Analysis of DUSP1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysateLane 2: Jurkat cell lysate Lane 3: mouse spleen tissue lysate](http://images.novusbio.com/images2/MKP-1-DUSP1-Antibody-(JJ0930)-Western-Blot-NBP2-67909-img0004.jpg)
![Immunohistochemistry-Paraffin: MKP-1/DUSP1 Antibody (JJ0930) [NBP2-67909] - Analysis of paraffin-embedded mouse lung tissue using anti-DUSP1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/MKP-1-DUSP1-Antibody-(JJ0930)-Immunohistochemistry-Paraffin-NBP2-67909-img0003.jpg)
![Flow Cytometry: MKP-1/DUSP1 Antibody (JJ0930) [NBP2-67909] - Analysis of HepG2 cells with DUSP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/images2/MKP-1-DUSP1-Antibody-(JJ0930)-Flow-Cytometry-NBP2-67909-img0001.jpg)
![Flow Cytometry: HLA DQ Antibody (SPV-L3) [NBP2-45041] - Flow Cytometric Analysis of Raji cells. HLA DQ Antibody (SPV-L3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/images2/HLA DQ Antibody (SPV-L3)-Flow Cytometry-NBP2-45041-img0001.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY licence.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0018.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0013.jpg)
![Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Immunohistochemistry-Frozen-NB100-524-img0016.jpg)
![Western Blot: Complement C4b/d Antibody (16D2) [NB200-541] - Western blot analysis of C4 expression in J774.A1 (A) aand mouse PBL (B) whole cell lysates.](http://images.novusbio.com/images2/Complement-C4b-d-Antibody-(16D2)-Western-Blot-NB200-541-img0001.jpg)
![Western Blot: ARC/NOL3 Antibody [NBP2-19561] - Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE diluted at 1:1000](http://images.novusbio.com/images2/ARC-NOL3-Antibody-Western-Blot-NBP2-19561-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ARC/NOL3 Antibody [NBP2-19561] - Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using NOL3 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.](http://images.novusbio.com/images2/ARC-NOL3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19561-img0005.jpg)
![Immunohistochemistry-Frozen: ARC/NOL3 Antibody [NBP2-19561] - NOL3 antibody detects NOL3 protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse cerebellum. Green: NOL3 stained by NOL3 antibody diluted at 1:250. Red: NF-H, stained by NF-H antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/images2/ARC-NOL3-Antibody-Immunohistochemistry-Frozen-NBP2-19561-img0010.jpg)
![Western Blot: ARC/ARG3.1 Antibody [NBP1-56929] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:312500 Positive Control: Transfected 293T](http://images.novusbio.com/images2/ARC-ARG3.1 Antibody-Western Blot-NBP1-56929-img0010.jpg)
![Immunohistochemistry-Paraffin: ARC/ARG3.1 Antibody [NBP1-56929] - Human adrenal tissue at an antibody concentration of 5ug/ml.](http://images.novusbio.com/images2/ARC-ARG3.1-Antibody-Immunohistochemistry-Paraffin-NBP1-56929-img0004.jpg)
![Western Blot: ARC/ARG3.1 Antibody [NBP1-56929] - Analysis of 293T cell lysate. Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/images2/ARC-ARG3.1-Antibody-Western-Blot-NBP1-56929-img0005.jpg)
![Western Blot: IgM Antibody (IM260) [NBP2-34253] - Western Blot Analysis of Raji cell lysate using IgM Antibody (IM260)](http://images.novusbio.com/images2/IgM-Antibody-(IM260)-Western-Blot-NBP2-34253-img0005.jpg "Western Blot: IgM Antibody (IM260) [NBP2-34253] - Western Blot Analysis of Raji cell lysate using IgM Antibody (IM260)")