| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 555 | NB7494AFP555 | |||
| Alexa Fluor Plus 594 | NB7494AFP594 | |||
| Alexa Fluor Plus 647 | NB7494AFP647 | |||
| Alexa Fluor Plus 680 | NB7494AFP680 | |||
| Allophycocyanin | NB7494APC | |||
| FITC | NB7496 | |||
| HRP | NB7497 | |||
| Janelia Fluor 525 | NB7494JF525 | |||
| Janelia Fluor 669 | NB7494JF669 | |||
| PE | NB7494PE | |||
| PerCP | NB7494PCP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NBP1-75025 | |||
| Biotin | NBP1-75026 | |||
| FITC | NBP1-75027 | |||
| HRP | NBP1-75028 | |||
![Flow Cytometry: Goat anti-Feline IgM Heavy Chain Secondary Antibody [NB7258] - The cat cells were washed and Incubated with the IgM antibody. It was used to detect Bcells. This image was submitted via customer review. Image from the FITC version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Feline-IgM-Secondary-Antibody-Flow-Cytometry-NB7258-img0001.jpg "Flow Cytometry: Goat anti-Feline IgM Heavy Chain Secondary Antibody [NB7258] - The cat cells were washed and Incubated with the IgM antibody. It was used to detect Bcells. This image was submitted via customer review. Image from the FITC version of this antibody.")
![Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [NB120-9167] - Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgM Secondary Antibody [DyLight 488] [NBP1-72873] Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgM-Heavy-Chain-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NB120-9167-img0001.jpg "Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [NB120-9167] - Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgM Secondary Antibody [DyLight 488] [NBP1-72873] Image from the DyLight 488 version of this antibody.")
![Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [NB120-9167] - Image using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgM-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NB120-9167-img0002.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [NB120-9167] - Image using the DyLight 680 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgM-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NB120-9167-img0004.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [NB120-9167] - Image using the DyLight 800 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgM-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NB120-9167-img0006.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Mouse IgM Heavy Chain Secondary Antibody [NB120-9167] - Image using the DyLight 488 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgM-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NB120-9167-img0007.jpg)
![Western Blot: Goat anti-Rat IgM Heavy Chain Secondary Antibody [NBP2-60672] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher. Image using the Biotin form of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rat-IgM-Heavy-Chain-Secondary-Antibody-Western-Blot-NBP2-60672-img0011.jpg "Western Blot: Goat anti-Rat IgM Heavy Chain Secondary Antibody [NBP2-60672] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher. Image using the Biotin form of this antibody.")
![Immunocytochemistry/Immunofluorescence: Goat anti-Rat IgM Heavy Chain Secondary Antibody [NBP2-60672] - Anti-histone detection using a DyLight 488 conjugate (green). Anti-Tubulin was detected using a DyLight 549 conjugate (red). Nuclei were counter-stained using DAPI (blue). The image was captured using an Axio Imager.Z1 (Zeiss Micro Imaging Inc). Image using the DyLight 488 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rat-IgM-Heavy-Chain-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-60672-img0007.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Rat IgM Heavy Chain Secondary Antibody [NBP2-60672] - Image using the DyLight 488 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rat-IgM-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60672-img0008.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Rat IgM Heavy Chain Secondary Antibody [NBP2-60672] - Image using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rat-IgM-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60672-img0012.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Rat IgM Heavy Chain Secondary Antibody [NBP2-60672] - Image using the DyLight 800 format of this antibody.](http://images.novusbio.com/fullsize/Goat anti-Rat IgM Heavy Chain Secondary Antibody-Fluorophore-linked immunosorbent assay-NBP2-60672-img0014.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Rat IgM Heavy Chain Secondary Antibody [NBP2-60672] - Image using the DyLight 680 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Rat-IgM-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60672-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Goat anti-Human IgM Heavy Chain Secondary Antibody [NBP2-60670] - Immunocytochemistry/Immunofluorescence: Goat anti-Human IgM Secondary Antibody [DyLight 488] [NBP1-72860]](http://images.novusbio.com/fullsize/Goat-anti-Human-IgM-Heavy-Chain-Secondary-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-60670-img0002.jpg "Immunocytochemistry/Immunofluorescence: Goat anti-Human IgM Heavy Chain Secondary Antibody [NBP2-60670] - Immunocytochemistry/Immunofluorescence: Goat anti-Human IgM Secondary Antibody [DyLight 488] [NBP1-72860]")
![Fluorophore-linked immunosorbent assay: Goat anti-Human IgM Heavy Chain Secondary Antibody [NBP2-60670] - Image using the DyLight 405 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Human-IgM-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60670-img0003.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Human IgM Heavy Chain Secondary Antibody [NBP2-60670] - Image using the DyLight 488 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Human-IgM-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60670-img0004.jpg)
![ELISA: Goat anti-Human IgM Heavy Chain Secondary Antibody [NBP2-60670] - Image using the DyLight 680 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Human-IgM-Heavy-Chain-Secondary-Antibody-ELISA-NBP2-60670-img0005.jpg)
![Fluorophore-linked immunosorbent assay: Goat anti-Human IgM Heavy Chain Secondary Antibody [NBP2-60670] - Image using the DyLight 800 format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Human-IgM-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP2-60670-img0008.jpg)
![ELISA: Goat anti-Human IgM Heavy Chain Secondary Antibody [NBP2-60670] - ELISA results of purified Goat anti-Human IgM Heavy Chain Secondary antibody tested against purified human IgM [RED line], human IgG F(ab')2 {PURPLE line], human IgG Fab [BLUE line], human IgG F(c) [GREEN line]. Each well was coated in duplicate with 1.0 ug of IgM, IgG F(ab')2, IgG Fab, IgG F(c). The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer, Donkey-anti-Goat IgG HRP conjugate 1:12, 000, and TMB substrate.](http://images.novusbio.com/fullsize/Goat-anti-Human-IgM-Heavy-Chain-Secondary-Antibody-ELISA-NBP2-60670-img0009.jpg)
![Western Blot: Goat anti-Monkey IgM Heavy Chain Secondary Antibody [NBP2-59719] - Western Blot of Goat anti-Monkey IgM Heavy Chain Secondary antibody. Lane 1: Monkey IgM. Lane 2: Monkey IgG.Lane 3: Monkey IgG (A and M Free).Lane 4: Monkey IgG F(c). Load: 50 ng per lane.Primary antibody: Goat anti-Monkey IgM Heavy Chain Secondary antibody at 1:1,000 for 1 hour at RT.Secondary antibody: Peroxidase conjugated Dk-a-Gt IgG at 1:40,000 for 30 min at RT.Block for 30 min at RT.Predicted/Observed size: 70 kDa, 70 kDa for Monkey IgM (mu chain).](http://images.novusbio.com/fullsize/Goat anti-Monkey IgM Heavy Chain Secondary Antibody-Western Blot-NBP2-59719-img0005.jpg "Western Blot: Goat anti-Monkey IgM Heavy Chain Secondary Antibody [NBP2-59719] - Western Blot of Goat anti-Monkey IgM Heavy Chain Secondary antibody. Lane 1: Monkey IgM. Lane 2: Monkey IgG.Lane 3: Monkey IgG (A and M Free).Lane 4: Monkey IgG F(c). Load: 50 ng per lane.Primary antibody: Goat anti-Monkey IgM Heavy Chain Secondary antibody at 1:1,000 for 1 hour at RT.Secondary antibody: Peroxidase conjugated Dk-a-Gt IgG at 1:40,000 for 30 min at RT.Block for 30 min at RT.Predicted/Observed size: 70 kDa, 70 kDa for Monkey IgM (mu chain).")
![Western Blot: Goat anti-Monkey IgM Heavy Chain Secondary Antibody [NBP2-59719] - Lane 1: Monkey IgM. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min t RT. Predicted/Observed size: 72 kDa, 72 kDa for Monkey IgM. Other band(s): None. Image using the Fluorescein form of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Monkey-IgM-Heavy-Chain-Secondary-Antibody-Western-Blot-NBP2-59719-img0003.jpg)
![N/A: Goat anti-Monkey IgM Heavy Chain Secondary Antibody [NBP2-59719]](http://images.novusbio.com/fullsize/Goat-anti-Monkey-IgM-Heavy-Chain-Secondary-Antibody-N-A-NBP2-59719-img0004.jpg)