Goat anti-Human IgM Heavy Chain Secondary Antibody (Pre-adsorbed)

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Format: Pre-adsorbed

Goat anti-Human IgM Heavy Chain Secondary Antibody (Pre-adsorbed) Summary

• Description: Purity > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
• Immunogen: Purified Human IgM, Mu chain
• Specificity: Based on IEP, this antibody reacts with heavy mu chains on human IgM. This antibody has been pre-adsorbed against human IgA + IgG.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IGHM
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Chemiluminescence Immunoassay
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunoassay
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunomicroscopy 1:10 - 1:500
  • Western Blot 1:100-1:2000

Reactivity Notes

Based on IEP, no reactivity is observed to non-immunoglobulin human serum proteins, light chains on all human immunoglobulins, and human IgA or IgG

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.05% Sodium Azide
• Purity: Affinity purified

Alternate Names for Goat anti-Human IgM Heavy Chain Secondary Antibody (Pre-adsorbed)

• Constant region of heavy chain of IgM
• Ig Mu Chain C Region BOT
• Ig Mu Chain C Region GAL
• Ig Mu Chain C Region OU
• immunoglobulin heavy constant mu
• Immunoglobulin mu chain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgM Antibody (NBP1-75017)(1)

Publication using NBP1-75017

• Sarno J, Domizi P, Liu Y et al. Dasatinib overcomes glucocorticoid resistance in B-cell acute lymphoblastic leukemia Nature communications 2023-05-22 [PMID: 37217509]

Bioinformatics

• Gene Symbol: IGHM