IgM Antibody (RM109) [Biotin]

ELISA: IgM Antibody (RM109) [Biotin] [NBP2-62012B] - ELISA of mouse immunoglobulins shows NBP2-62012B reacts to mouse IgM; no cross reactivity with IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with different immunoglobulins.NBP2-62012B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

ELISA: IgM Antibody (RM109) [Biotin] [NBP2-62012B] - A titer ELISA of mouse IgM. The plate was coated with different amounts of mouse IgM. A serial dilution of NBP2-62012B was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

• Reactivity: Mu
• Applications: ELISA
• Clone: RM109
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Biotin
• Concentration: 1 mg/ml

IgM Antibody (RM109) [Biotin] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Mouse IgM
• Specificity: This antibody reacts to mouse IgM. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, human IgG, rat IgG, or goat IgG.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IGHM
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 0.005 ug/ml - 0.2 ug/ml
• Application Notes: This primary antibody can also be used as a secondary antibody.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, 1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for IgM Antibody (RM109) [Biotin]

• AGM1
• DKFZp686I15196
• DKFZp686I15212
• FLJ00385
• IgM
• immunoglobulin heavy constant mu
• MGC104996
• MGC52291
• MU
• VH

Background

IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to its large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to its high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. IgM measurement yields information about the body's immediate resistance and response to infection as well as information related to specific diseases. Decreased levels are associated with immune deficiency states, hereditary deficiencies, and myeloma. Increased levels can be associated with Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGHM