Rabbit IgG Isotype Control [Alexa Fluor® 647]

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Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, ELISA, IHC, PAGE
Host
Rabbit
Conjugate
Alexa Fluor 647

Rabbit IgG Isotype Control [Alexa Fluor® 647] Summary

Description
This Rabbit IgG immunoglobulin is useful as an isotype control for Rabbit IgG antibody.
Specificity
Rabbit IgG whole molecular was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer. Rabbit IgG whole molecular was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Rabbit Serum.
Isotype
IgG
Host
Rabbit

Applications/Dilutions

Application Notes
Rabbit IgG whole molecule can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Reviewed Applications
Read 1 Review rated 3
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NBP2-36463AF647 in the following application:

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Borate.
Preservative
0.05% Sodium Azide

Alternate Names for Rabbit IgG Isotype Control [Alexa Fluor® 647]

  • IgG
  • immunoglobulin heavy constant gamma 1 (G1m marker)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Review for IgG Isotype Control (NBP2-36463AF647) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Other.
We have 1 review tested in 1 application: Flow.

Reviews using NBP2-36463AF647:
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Images Ratings Applications Species Date Details
Flow Cytometry IgG NBP2-36463AF647
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reviewed by:
Anonymous
Flow Other 02/26/2015
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Summary

ApplicationFlow Cytometry
Sample TestedMouse CD4+ T Cells
SpeciesOther
Lot31908-AF647

Blocking

Blocking DetailsNone

Primary Anitbody

Dilution Ratio1:100 dilution in 1X Permeabilization buffer (Foxp3 Staining Buffer Set from eBiosciences), 30 minutes incubation at 21°C

Details

Detection NotesLSR II flow cytometer (BD); NBP1-49533AF647 stained Mouse CD4+ T Cells as +ve Control and Fluorescence minus one/FMO control
Fixation DetailsSee Additional Comments
Permeabilization DetailsCells fixed and permeabilized with Foxp3 Staining Buffer set (eBioSciences #00-5523-00) for intracellular staining
Wash DescriptionOne wash with 1X Permeabilization buffer (eBiosciences) and one wash with FACS buffer (1x PBS, 1% BSA, 0.1% sodium azide)

Comments

CommentsMurine spleens were harvested and CD4+ T cells were isolated via negative selection. CD4+ T cells were stimulated with anti-CD3/CD28 beads and insulin (1μg/mL) for 5 days in culture media with additional glucose provided. Cells were washed and beads removed prior to staining. Cells were stained for surface antigens, washed, then fixed and permeabilized (via Foxp3 Staining Buffer set (eBioSciences #00-5523-00)) for intracellular staining (Novus informed me that the epitope is intracellular for this antibody). Cells were washed again before being ran on LSR II Flow Cytometer. Data was analyzed via FlowJo (Tree Star). Cells were gated, in order, on lymphocytes, single cells, live cells, and CD4+ cells. Positive controls and both isotype and FMO controls were compared.

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Anonymous
02/26/2015
Application: Flow
Species: Other