Monkey IgG Isotype Control

Western Blot: Monkey IgG Isotype Control [NBP1-97047] - SDS-PAGE of Monkey IgG Whole Molecule. Lane 1: Non-Reduced Monkey IgG Whole Molecule. Lane 2: 5 uL Opal Prestained Marker . Lane 3: Reduced Monkey IgG Whole Molecule. Load: 1 ug per lane. Predicted/Observed size: Non-Reduced at 160 kDa, Observed at greater than 180; Reduced at 55, 25 kDa. Non-reduced sample migrates higher due to large molecular weight.

• Reactivity: Pm
• Applications: WB, ELISA, IHC, PAGE
• Host: Monkey
• Conjugate: Unconjugated
• Format: Purified
• Concentration: LYOPH

Monkey IgG Isotype Control Summary

• Description: This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Monkey IgG and anti-Monkey Serum.
  
  Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
• Isotype: IgG
• Host: Monkey
• Gene: IGHG1
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry 1:10 - 1:500
  • SDS-Page
  • Western Blot 1:100 - 1:2000
• Application Notes: This product has been tested by SDS-Page and can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20C or below for long term storage.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 500 ul deionized water (or equivalent).

Alternate Names for Monkey IgG Isotype Control

• IgG
• Immunoglobulin G
• ImmunoglobulinG

Background

IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG Isotype Control (NBP1-97047)(1)

Publication using NBP1-97047

• Ros-Ganan I, Hommel M, Trigueros-Motos L et al. Optimising the IgG-degrading enzyme treatment regimen for enhanced adeno-associated virus transduction in the presence of neutralising antibodies Clinical & Translational Immunology 2022-01-01 [PMID: 35228870]

FAQs for IgG Isotype Control (NBP1-97047). (Showing 1 - 1 of 1 FAQ).

1. What is the species of monkey used to generate this IgG (old world, new world?)
   • This product was prepared from a mixture of old world monkey species, including Cyno, Rhesus, etc. We can also make it from one species through custom order. 

Bioinformatics

• Gene Symbol: IGHG1