Human IgG Isotype Control

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Western Blot: Human IgG Isotype Control [NBP1-97043] - SDS-PAGE of Human IgG.Lane 1: Reduced Human IgG.Lane 2: 5uL OPAL Pre-stained Marker MB-210-0500. Lane 3: Non-reduced Human IgG.Load: 1ug per lane.Predicted/Observed ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow
Host
Human
Conjugate
Unconjugated
Concentration
LYOPH

Human IgG Isotype Control Summary

Description
This Human IgG immunoglobulin is useful as an isotype control for Human IgG antibody.
Isotype
IgG
Host
Human
Purity
Ion exchange chromatography

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:5000
  • ELISA 1:10000 - 1:50000
  • Flow Cytometry 1:500

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Ion exchange chromatography
Reconstitution Instructions
Reconstitute with 1.0 ml deionized water.

Alternate Names for Human IgG Isotype Control

  • IgG
  • immunoglobulin heavy constant gamma 1 (G1m marker)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains...  Read full blog post.

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