Rat IgG Isotype Control [FITC]

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications FLISA, Flow, IHC-P, ICC
Clonality
Polyclonal
Host
Rat
Conjugate
FITC
Concentration
Please contact technical services for concentration.

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Catalog# & Conjugate Size Price

Rat IgG Isotype Control [FITC] Summary

Description
This Rat IgG immunoglobulin is useful as an isotype control for Rat IgG antibody.
Isotype
IgG
Clonality
Polyclonal
Host
Rat

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10 - 1:1000
  • Fluorophore-linked immunosorbent assay 1:10 - 1:2000
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunocytochemistry 1:10 - 1:500
Application Notes
Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS.
Preservative
Sodium Azide
Concentration
Please contact technical services for concentration.

Alternate Names for Rat IgG Isotype Control [FITC]

  • IgG
  • immunoglobulin heavy constant gamma 1 (G1m marker)

Background

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10^11 variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains...  Read full blog post.

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Concentration Calculator

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