Rat IgG Isotype Control

Flow Cytometry: Rat IgG Isotype Control [NBP1-71666] - Image from verified customer review.

ELISA: Rat IgG Isotype Control [NBP1-71666] - ELISA plate was coated with serially diluted Rat IgG-UNLB. Immunoglobulin was detected with Goat Anti-Rat IgG-BIOT and Mouse Anti-Rat IgM-BIOT followed by Streptavidin-HRP and quantified.

• Reactivity: Rt
• Applications: ELISA, Flow, IP
• Clonality: Polyclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: Please contact technical services for concentration.

Rat IgG Isotype Control Summary

• Description: This Rat IgG immunoglobulin is useful as an isotype control for Rat IgG antibody.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rat
• Gene: IGHG1

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Fluorophore-linked immunosorbent assay
  • Immunoprecipitation 1:10 - 1:500
• Application Notes: IP usage was reported in (PMID: 22065344) Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: BBS, pH 8.2.
• Preservative: No Preservative
• Concentration: Please contact technical services for concentration.

Alternate Names for Rat IgG Isotype Control

• IgG
• Immunoglobulin G
• ImmunoglobulinG

Background

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10^11 variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG Isotype Control (NBP1-71666)(1)

Publication using NBP1-71666

• Wang Y, Taylor TH, Arriaga EA. Analysis of the bioactivity of magnetically immunoisolated peroxisomes. Anal Bioanal Chem;402(1):41-9. 2012-01-01 [PMID: 22065344] (IP)

Review for IgG Isotype Control (NBP1-71666) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Verified Customer 05/16/2018

Summary

• Lot: E0917-P188

Bioinformatics

• Gene Symbol: IGHG1