Rabbit IgG Isotype Control [Agarose]

Product Discontinued

Rabbit IgG Isotype Control [Agarose] Summary

• Description: This Rabbit IgG immunoglobulin is useful as an isotype control for Rabbit IgG antibodies.
• Kit Type: Kit
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IGHG1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
• Application Notes: Rabbit IgG was coupled to agarose beads using a cyanogen bromide method. Rabbit IgG concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG, before coupling to agarose beads. Useful as a positive control in immunoassays. IP control that is used in conjunction with agarose-immobilize antibodies made in rabbit. WB usage was reported in scientific literature (PMID: 24569374). Proximity Ligation Assay usage was reported in scientific literature (PMID: 24569374). Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: 2 - 8C / 2 years from date of receipt
• Buffer: PBS.
• Preservative: 0.1% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Protein concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Alternate Names for Rabbit IgG Isotype Control [Agarose]

• IgG
• Immunoglobulin G
• ImmunoglobulinG

Background

IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG Isotype Control (NB600-441)(2)

Publications using NB600-441

• Ma XH, Piao SF, Dey S et al. Targeting ER stress-induced autophagy overcomes BRAF inhibitor resistance in melanoma. J Allergy Clin Immunol. [PMID: 24569374] (PLA, WB, Human)
  
  Details:
  Citation using the Agarose Immobilized form of this antibody.
• Schiller, N et al. Participation of macrophages in atherosclerotic lesion morphology in LDLr-/- mice. Journal of Lipid Research. [PMID: 15175354]
  
  Details:
  Citation using the Agarose Immobilized form of this antibody.

Bioinformatics

• Gene Symbol: IGHG1