Armenian Hamster IgG Isotype Control (299Arm)

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Product Details

Summary
Reactivity ArHaSpecies Glossary
Applications ELISA, Flow, ICC/IF, IHC, In vitro, Ctrl
Clone
299Arm
Clonality
Monoclonal
Host
Armenian Hamster
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Armenian Hamster IgG Isotype Control (299Arm) Summary

Isotype
IgG
Clonality
Monoclonal
Host
Armenian Hamster
Purity
Affinity purified

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
Application Notes
This product is useful as an isotype control immunoglobulin This product has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood. Use isotype control at the same concentration as experimental antibody..

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for Armenian Hamster IgG Isotype Control (299Arm)

  • IgG
  • immunoglobulin heavy constant gamma 1 (G1m marker)

Background

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for IgG Isotype Control (NBP2-59616) (0)

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